摘要
从NCBI数据库中检索大肠杆菌MG1655膜蛋白pspD、yiaW和yeeE的氨基酸序列，采用生物信息学在线分析软件对三种膜蛋白的理化性质、保守结构域、跨膜区、信号肽、磷酸化位点、糖基化位点及相互作用蛋白拓扑网络进行了预测分析。实验表明pspD为亲水性蛋白，分子中不存在跨膜结构，yiaW和yeeE为疏水性蛋白，分子中分别有2个和9个跨膜区；3个蛋白分子中均不存在信号肽序列，也没有磷酸化位点，pspD中有1个糖基化位点，yiaW和yeeE中分别有2个和7个糖基化位点。3个蛋白二级结构中组成最多的是α-螺旋分别占56．16％、57．94％$1142．61％。三级结构的预测结果与二级结构预测一致。对其相互作用蛋白的拓扑网络预测发现，pspD属于噬菌体休克蛋白操纵子家族成员，与pspA、pspB和pspC蛋白关系最为密切，推测其可能在特殊环境中对于维持膜功能有极其重要的作用；yiaW与yiaV蛋白为膜融合蛋白（外排泵组件，信号锚，与物质外排有关），和ycdZ（DUF1097家族内膜蛋白）、nrfA（亚硝酸还原酶）、nrfD（甲酸依赖亚硝酸盐还原酶）蛋白相互作用关系最为密切。yeeE蛋白与保守蛋白yeeD和yedF为同源蛋白，关系最为密切。此外，yeeE蛋白与cysJ、Cysp、cysN、cysD等硫酸盐跨膜运输蛋白关系密切，根据前面的预测其有9个跨膜区，推测其可能与物质的跨膜转运相关。
In this research, the amino acid sequences ofpspD, yiaW and yeeE protein ofEscherichia coli MG1655 cell, membrane were retrievaled from the NCBI database. Physicochemical properties, Conserved domains, transmembrane, signal peptide, phosphorylation, glycosylation sites and interacting protein of these proteins were predicted and analyzed in online analysis softwares. The results showed that pspD was a hydrophilic protein, transmembrane structure did not exist in the molecule, yiaW and yeeE was the hydrophobic protein, and there was respectively 2 and 9 transmembrane regions in molecules; three proteins were all not found the signal peptide sequence and phosphorylation sites. Molecules of pspD yiaW, and yeeE were 1,2 and 7 glycosylation sites, respectively. The two stage structures of pspD,yiaW, and yeeE protein, alpha helix was the most important component, accounting for 56.16%, 57.94% and 42.61% respectively. The predict outcomes of the three stage structure was consistent with the predict outcomes of the two stage structure. The topology network prediction showed that pspD belonged to the phage shock protein operon family, and it was most closely related to pspA, pspB and pspC, may played an extremely important role in maintaining the membrane function in the special environment; yiaW and yiaV protein was a membrane fusion protein （MFP） （efflux pump assembly, signal anchor, and material the row）. The interaction with ycdZ （DUF1097 family ofendometrial proteins）, nrfA （nitrite reductase） and nrfD （formate dependent nitrite reductase） protein is most closely related, yeeE was homologous with conserved protein ofyeeD and yedF, they were most closely related. In addition, yeeE was closely related to the transmembrane transport proteins of cysJ, Cysp, cysN, cysD and so on, according to the previous prediction of 9 transmembrane regions that it might be related to transmembrane transport of substance.
作者
王永刚
杨光瑞
冷非凡
杨明俊
王鸣刚
陈凯
Wang Yonggang;Yang Guangrui;Leng Feifan;Yang Mingiun;Wang Minggang;Chen Kai(Lanzhou University of Technology,School of Life Science and Engineering,Lanzhou,730050;Lanzhou University of Technology,School of Petrochemical Engineering,Lanzhou,730050)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第11期4763-4768,共6页
Genomics and Applied Biology
基金
国家自然科学基金项目资助(No.31460032
81660581)
