摘要
目的探讨KLF2转录因子在RDS大鼠肺组织的表达以及与甲状腺素水平的关系,为肺泡表面活性物质治疗不敏感的NRDS提供可能的理论依据。方法注射油酸建立的RDS大鼠模型为研究组,分别于注射后3 h、18 h、24 h用电化学发光法检测血清甲状腺素水平,用Western blot法检测肺组织中KLF2蛋白的表达,并与非RDS大鼠做对照。结果油酸3 h组、18 h组中的血清FT4与正常对照组比较差异有统计学意义(P<0. 01);油酸3 h组中T4与正常对照组比较差异有统计学意义(P<0. 01);油酸18 h组中T4与正常对照组比较差异有统计学意义(P<0. 05)。肺组织中的KLF2蛋白量表达油酸3 h组与正常对照组比较差异有统计学意义(P<0. 05),而18 h组、24 h组与正常对照组差异无统计学意义(P>0. 05)。结论呼吸窘迫综合症与血清甲状腺素水平、KLF2蛋白的表达有相关性,FT4、T4与KLF2有相关性。
Objective To investigate the expression of KLF2 transcription factor in lung tissue of RDS rats and its relationship with thy- roxine level. Methods The RDS rats models （ study group） induced by injecting acid were detected the serum level of thyroxine by Electro- chemiluminescence after injection 3 h, 18 h, 24 h, and the expression of KLF2 protein in lung tissue was tested by Western blot, non-liDS rats were as contral group. Results The serum levels of FT4, T4 were significant differences between 3 h, 18 h study groups and normal groups （P〈0. 01, P〈0. 05 ）. The expression of KLF2 protein in lung tissue was significantly difl＇erent between the 3 h study group and control group, and there were no significant differences between the 18 h, 24 h study groups and control groups. Conclusion Respiratory distress syndrome is related to the serum levels of thyroxine and KLF2 protein, and FF4, I＂4 is related to KLF2.
作者
钟红平
陈彦香
刘世平
姜泓
ZHONG Hong-Ping;CHEN Yan-Xiang;LIU Shi-Ping(Department of Pediatrics,Affiliated Hospital of Yah＇an University,Yan＇an,Shaanxi 716000,China)
出处
《中国妇幼保健》
CAS
2018年第21期4978-4980,共3页
Maternal and Child Health Care of China
基金
宁夏回族自治区自然科学基金项目(NZ13205)
