摘要
选取2018年3月16日河南大学附属郑州颐和医院发现的1例新生儿溶血病(HDN)患儿作为观察对象,对患儿进行ABO与Rh(D)血型鉴定、直接抗人球蛋白试验、游离试验和放散试验,采用微柱凝胶抗人球蛋白法筛查患儿血清不规则抗体,鉴定抗体特异性,进行MN分型;最后制备NN型反定细胞重新检测游离抗体试验,记录各试验结果并进行分析,探讨抗M抗体对HDN实验室检测结果的影响。该患儿血清中检测出抗M抗体,血型鉴定结果为B型Rh(D)阳性,MN分型结果为NN型,直接抗人球蛋白试验结果呈阴性,游离试验结果呈阳性,NN型反定细胞游离试验结果呈阳性(凝集强度减弱),放散试验结果呈阳性。该患儿为ABO系统引起的HDN,游离抗体试验受到抗M抗体的干扰。
A case of hemolytic disease of a newborn（HDN） was found in Zhengzhou Yihe Hospital Affiliated to Henan University on March 16, 2018. ABO blood typing, Rh（D） blood typing, Coombs＇ test（direct anti-human globulin test）, free antibody test and release test were performed for the newborn. Microcolumn gel anti-human globulin method was used to screen the irregular antibodies in serum, identify the specificity of antibodies, and carry out MN typing; finally, NN-type negative cells were prepared to re-detect the free antibody test, and the above various results were recorded and analyzed to investigate the effect of anti M antibody on laboratory test result of HDN. Anti M antibody was detected in the serum of the newborn. The blood group identification result was B type Rh（D） positive, the MN typing result was NN type, Coombs＇ test presented negative, free antibody test positive, release test positive; the results of NN type reverse cell free test were positive（the agglutination intensity was weakened）. The cause of the newborn hemolytic disease is of ABO blood group incompatibility, and the free antibody trials are interfered by anti M antibody.
作者
王丽娜
张勤
闫烨
吕毅
Wang Lina;Zhang Qin;Yan Ye;Lyu Yi(Clinical Laboratory Medicine Center,Zhengzhou Yihe Hospital Affiliated to Henan University,Zhengzhou 450047,Henan,China)
出处
《实用检验医师杂志》
2018年第3期182-183,186,共3页
Chinese Journal of Clinical Pathologist
关键词
新生儿溶血病
MNS血型系统
游离抗体
Hemolytic disease of newborn
MNS blood group system
Free antibody
