摘要
目的:研究NF-κB信号转导通路与复发性口腔溃疡(RAU)易感性的相关性。方法:采用ELISA方法检测124例RAU患者和133例健康对照者中NFκBp50、NFκBp65、IκBα、IKK的血清含量,则用限制性片段长度多态性-聚合酶链式反应(RFLP-PCR)进行NFκB-94 ins/del ATTG位点的基因多态性分析,用比值比(OR)和95%可信区间(95%CI)估计相对危险度,采用SPSS 20.0软件包对数据进行统计学分析。结果:RAU组患者血清NFκBp50、NFκBp65、IKK水平均显著低于正常对照组(P<0.05),RAU组患者血清IκBα水平均显著高于正常对照组(P<0.05)。NFκB-94ins/del ATTG位点在基因型频率与等位基因频率的分布上,病例组与正常对照组之间均有显著差异(P<0.05)。NFκB-94 ins/del ATTG位点基因型ID(OR=3.073,95%CI=1.557-6.067)与DD(OR=4.851,95%CI=2.264-10.393)为高风险基因型,D等位基因为高风险等位基因(OR=2.079,95%CI=1.462-2.957)。结论:NF-κB信号转导通路与复发性口腔溃疡有一定关系。NFκB-94 ins/del ATTG位点是RAU患者的易感基因位点。NFκB-94 ins/del ATTG位点携带D等位基因,患RAU的风险性是携带I等位基因的2.079倍。
PURPOSE： To explore the possible relationship between recurrent aphthous ulcer（RAU） and nuclear factor-κB signaling pathway. METHODS： A total of 124 RAU patients were recruited for this study. The control group consisted of 133 healthy subjects. Serum NFκBp50, NFκBp65, IκBα and IKK concentration were detected by ELISA.NFκB-94 ins/del ATTG sites were detected by restriction fragment length polymorphism-polymerase chain reaction（RFLP-PCR）.Relative risk ratios were estimated by odds ratios（OR） and 95% confidence interval（95% CI）. Statistical analysis was performed using SPSS 20.0 software package. RESULTS： Serum NFκBp50, NFκBp65 and IKK levels in RAU patients were significantly lower than those of the controls（P〈0.05）. Serum IκBα level in RAU patients was significantly higher than those of the controls（P〈0.05）. Significant differences were found in the genotype frequencies or allele frequencies of NFκB-94 ins/del ATTG sites between RAU patients and controls（P〈0.05）. ID genotype（OR =3.073,95% CI =1.557-6.067）, DD genotype（OR=4.851,95%CI=2.264-10.393）, and D allele（OR=2.079,95%CI=1.462-2.957） at NFκB-94 ins/del ATTG site exhibited high risks of RAU. CONCLUSIONS： NF kappa B signaling pathway is associated with RAU.NFκB-94 ins/del ATTG sites are associated with higher risk of RAU. NFκB-94 ins/del ATTG D allele may serve as genetic determinants for RAU.
作者
张敬
令狐瑞琦
沙晶晶
胡欣培
李丹
李梦源
ZHANG Jing;LINGHU Rui-qi;SHA Jing-jing;HU Xin-pei;LI Dan;LI Meng-yuan(Department of Endodontics,Stomatological Hospital of Ningxia Medical University General Hospital.Yinchuan 750004,Ningxia Hui Autonomous Region,China;Rabbit Dental Group,Jixiang Branch of Xi＇an City.Xi＇an 710077,Shaanxi Province,China;Shimane University Medical Department.Izumo 6938501,Japan;Graduate School of Ningxia Medical University.Yinchuan 750004,Ningxia Hui Autonomous Regions,China)
出处
《上海口腔医学》
CAS
CSCD
北大核心
2018年第5期513-517,共5页
Shanghai Journal of Stomatology
基金
宁夏医科大学校级科研重点项目(XZ2016014)
