摘要
以曲茎石斛组培苗为实验材料,探讨了不同培养基、激素配比、继代周期和活性炭浓度,对于其不定芽增殖和生根的影响.结果表明,培养基B5+0.3mg/L 6-BA(6-苄基腺嘌呤)+0.03mg/L NAA(萘乙酸)+6g/L琼脂粉+30g/L蔗糖促进组培苗增殖的效果较好.在30d、50d、70d继代周期的培养试验中,50d的增殖效果较好;添加0.30mg/L NAA、0.8g/L活性碳的培养基对组培苗生根的促进作用较好.试验结果为曲茎石斛组培苗的大规模快繁提供了数据支持.
The effects of different culture medium,growth hormone proportion,subculture cycle and concentration of active carbon on multiplication of adventitious buds,rooting of Dendrobium flexicaule Z.H.Tsi,S.C.Sun et L.G.Xu were researched in this experiment,in which the tissue culture seedlings were used as material for experiment.The results showed that the multiplication of adventitious buds was relatively satisfactory when the tissue culture seedlings were cultured in medium of B5＋0.3 mg/L 6-BA ＋0.03 mg/L NAA ＋6 g/L Agar＋30 g/L Sugar.In the subculture cycle experiment of 30 d,50 dand 70 d,subculture cycle of 50 dwas best in favor of multiplication of adventitious buds.When the medium was added with0.30 mg/L NAA and 0.8 g/L activated carbon,it drastically promoted the rooting of tissue culture seedlings in Dendrobium flexicaule Z.H.Tsi,S.C.Sun et L.G.Xu.The above experimental results statistically provided a technical basis for largescale production of Dendrobium flexicaule Z.H.Tsi,S.C.Sun et L.G.Xu.
作者
张明月
黄作喜
李强
ZHANG Mingyue;HUANG Zuoxi;LI Qiang(College of Life Science,Neijiang Normal University,Neijiang,Sichuan 641199,China;Sichuan Qiancao Bio technology Co.,Ltd.Neijiang,Sichuan 641000,China)
出处
《内江师范学院学报》
2018年第10期76-79,共4页
Journal of Neijiang Normal University
关键词
曲茎石斛
组培技术
增殖
生根
Dendrobium flexicaule Z. H. Tsi
S. C. Sun et L. G. Xu
tissue culture technology
multiplication
rooting
