摘要
采用荧光光谱法检测血糖。结果表明,检测血清的最佳激发波长为340 nm,血清中葡萄糖的发射峰位置为470 nm。随着血清中葡萄糖浓度的增加,荧光光谱图中荧光峰处荧光强度,面积积分强度整体呈上升趋势,荧光峰的半峰全宽整体呈现下降趋势。利用近红外光谱法检测血糖,血清可分辨的光谱区域位于5 600~6 060 cm-1范围内,不同血糖浓度的吸收峰均位于5 768 cm-1处。随着血糖浓度的增大,其吸收峰处的吸光度逐渐增大,面积积分强度也呈上升趋势。分析二者的优缺点,荧光分析法受外界因素的干扰更小,精确度更高,光谱参数与血糖浓度之间的规律更明显,在有创检测方面具有更明显的优势,而近红外光谱法检测血糖则在无创血糖检测方面具有更大的潜力。
Fluorescence spectroscopy was used to detect blood glucose. The results showed that the optimal excitationwavelength for detecting serum was 340 nm, and the emission peak position of glucose in serum was 470 nm. With theincrease of glucose levels, the fluorescence intensity at the fluorescence peak in the fluorescence spectrum and the areaintegrals showed an upward trend overall, and the full width at half maximum of the fluorescence peak showed adecreasing trend as a whole. Near-infrared spectroscopy was used to detect blood glucose. The resolvable spectra at5 600 ~ 6 060 cm-1, and the absorption peak of different glucose concentrations were located at 5 768 cm-1. With theincrease of glucose concentration, the absorbance at the absorption peak and the area integrals show an upward trend.Analyzing the advantages and disadvantages of the two, the fluorescence analysis method is less affected by externalfactors, and its accuracy was higher, the correlation between spectral parameters and blood glucose concentration is moreobvious, therefore it has more advantages in invasive detection, and near-infrared spectroscopy detection of blood glucosehas greater potential in non-invasive glucose detection.
作者
陶建峰
陈明惠
郑刚
王成
宋云霄
TAO Jianfeng;CHEN Minghui;ZHENG Gang;WANG Cheng;SONG Yunxiao(Shanghai Institute for Minimally Invasive Therapy,Institute for Medical Optics and Optometry,Shanghai 200093,China;Xuhui Central Hospital of Shanghai,Shanghai 200031,China)
出处
《激光生物学报》
CAS
2018年第5期428-434,共7页
Acta Laser Biology Sinica
基金
国家自然科学基金(61308115)
上海市自然科学基金(13ZR1457900)
上海市科委产学研医项目(15DZ1940400)
关键词
光谱学
检测
光谱分析
血糖
spectroscopy
detection
spectral analysis
blood glucose
