摘要
本研究旨在观察重组人源胶原蛋白(recombinant human collagen,r-hc)对小鼠皮肤激光损伤的修复作用,初步探索其作用机制。应用458~514 nm激光照射小鼠背部皮肤制作皮肤损伤模型,将r-hc外涂于创伤皮肤,剂量为8 mg/mL(生理盐水配制),每天涂抹1次,连续给药14 d。分别于给药后1、4、7、14 d用双光子显微镜收集二次谐波(second harmonic generation,SHG)信号检测伤口真皮中的胶原纤维,并进行常规HE染色,观察伤口局部的病理学改变。体外试验检测r-hc对人皮肤角质形成细胞和成纤维细胞增殖活力的影响,计算细胞存活率。在小鼠模型上显示,与对照组相比,r-hc可明显加速伤口的愈合,缩短伤口愈合时间; SHG显示r-hc能够促进创伤局部胶原的产生;体外试验也显示它有促进人皮肤成纤维细胞和角质形成细胞增殖的能力。由此可见,r-hc(8 mg/mL)对小鼠激光损伤皮肤有修复作用,可能是通过促进表皮角质细胞和真皮成纤维细胞的增殖,促进胶原的沉积而发挥修复作用的。
This study was to observe the repair effect of recombinant human collagen（ r-hc） on laser induced damage ofmice skin and to explore preliminarily its mechanism. Dorsal skin of mice was irradiated with 458 ~ 514 nm laser, r-hcdissolved in physiological saline solution（8 mg / mL） was used to treat dorsal skin wounds of mice, once per day for14 days. The collagen fibers in the wound dermis were observed by using the backwards second harmonic generation（SHG） signals which were collected by two photon microscopy（1,4,7,14 d） after the administration. The pathologicalchanges of the wound were observed by routine HE staining. The effects of r-hc on proliferation of epidermic cells and fi-broblasts were studied in vitro, and cell viability was calculated. The results indicated that compared with the controlgroup, r-hc significantly accelerated wound healing and shortened wound healing time in mice. SHG imaging showed thatr-hc could promote the production of collagen in wound, and in vitro experiments r-hc showed markedly proliferation-pro-moting activity for human skin fibroblasts and keratinocytes. It can be concluded that r-hc（8 mg / mL） has a repairingeffect on laser-damaged mouse skin, which may be achieved by stimulating the proliferation of epidermal keratinocytesand dermal fibroblasts and promoting collagen deposition.
作者
马淑骅
孙娅楠
杨伟峰
孙桂荣
李松林
蒋盈宝
王岩
王毅
MA Shuhua;SUN Yanan;YANG Weifeng;SUN Guirong;LI Songlin;JIANG Yingbao;WANG Yan;WANG Yi(Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China;Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for MajorDiseases,Beijing 100700,China;Tianjin Iriya Biological Technology Co.Ltd,Tianjin 300399,China;Tianjin Zhongxin Pharmaceutical Group Corporation Ltd.Zhongxin Pharmaceutical Factory,Tianjin 300380,China)
出处
《激光生物学报》
CAS
2018年第5期399-406,共8页
Acta Laser Biology Sinica
基金
国家自然科学基金面上项目(81373884)
国家自然科学基金青年项目(81603285)
中国中医科学院基本科研业务费(ZZ2016011)
关键词
重组人源胶原蛋白
激光损伤
创伤愈合
二次谐波
recombinant human collagen
laser irradiation damage
wound healing
second harmonic generation
