摘要
以印度块菌-云南松菌根际土壤细菌为研究对象,研究其种群组成和结构特征。(1)稀释平板法分离得到印度块菌-云南松菌根际土壤细菌的纯培养菌株,对菌株的16S rRNA序列测序分析,对测序的菌株数量和得到的OTUs数量绘制物种累积曲线,当物种累积曲线趋于平缓时,对OTUs进行系统发育分析,揭示可培养细菌的种群组成和结构特征。(2)对印度块菌-云南松菌根际土壤细菌16S rRNA基因的V3—V4区进行高通量测序,分析全部细菌类群的种群组成和结构特征。(1)分离得到菌根际可培养细菌793株,分属于3个属的61个OTUs,其中假单胞菌属(Pseudomonas)序列占总序列的86%,不动杆菌属(Acinetobacter)序列占总序列的9.8%,链霉菌属(Streptomyces)序列占总序列的6.5%。假单胞菌是印度块菌-云南松菌根际土壤可培养细菌的绝对优势类群。(2)高通量测序得到菌根际细菌序列8937条,分属于20个门、198属、2073个OTUs。隶属于变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和酸杆菌门(Acidobacteria)的OTUs占总OTUs的65.9%,变形菌门、放线菌门和酸杆菌门细菌是印度块菌-云南松菌根际土壤细菌的优势细菌。隶属于黄杆菌属(Flavobacterium)、根瘤菌属(Rhizobium)和假黄色单胞菌属(Pseudoxanthomona)的OTUs占总OTUs的33%,黄杆菌属、根瘤菌属和假黄色单胞菌属细菌是印度块菌-云南松菌根际土壤细菌的优势属。印度块菌-云南松菌根际土壤可培养细菌多样性较低,假单胞菌属细菌占据绝对优势地位。印度块菌-云南松菌根际土壤细菌类群具有较高的多样性,物种种类丰富,优势菌群集中。
The bacterial diversity of the Tuber indicum × Pinus yunnanensis mycorrhizosphere was studied. The mycorrhizosphere in T. indicum × Pinus yunnanensis was collected in the field and the culturable bacteria were purified from the soil. The 16S rRNA genes of the purified bacteria were sequenced and a phylogenetic tree was constructed to analyze the population composition and structure of the culturable bacteria. Roche 454 high-throughput sequencing was used to determine the population composition and structure of all bacteria in the soil. （ 1 ） 793 culturable bacteria, representing 3 geni and 61 OTUs （Operational Taxonomic Units ）, were purified from the mycorrhizosphere. OTUs of the genus Pseudomonas accounted for 86% of the total OTUs, OTUs of the genus Acinetobacter accounted for 9.8% of the total OTUs,and OTUs of the genus Streptomyces accounted for 6.5% of the total OTUs. Pseudomonas was the predominant cuhurable bacteria in the mycorrhizosphere of T. indicum × Pinus yunnanensis. （2） 8937 sequences of the bacterial V1--V3 region, representing 2073 OTUs, were analyzed by using Roche 454 high-throughput sequencing. Proteobacteria, Actinobacteria, and Acidobacteria were the three dominant phylums, which accounted for 65.9% of the total OTUs. Flavobacterium, Rhizobium, and Pseudoxanthomona were the dominant geni, which accounted for 33% of the total OTUs. The population composition of cuhurable bacteria of T. indicum × P. yunnanensis mycorrhizosphere was simple; the dominant genus was Pseudomonas. The bacterial diversity of T. indicum × P. yunnanensis mycorrhizosphere was very high; the bacterial population was complex.
作者
邓晓娟
闫兴富
刘建利
刘培贵
DENG Xiaojuan;YAN Xingfu;LIU Jianli;LIU Peigui(College of Biological Science and Engineering,Beifang University of Nationalities,Yinchuan 750021,China;Key Laboratory of Biodiversity and Biogeography,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China)
出处
《生态学报》
CAS
CSCD
北大核心
2018年第19期7066-7074,共9页
Acta Ecologica Sinica
基金
北方民族大学重点科研项目(2017KJ27)
北方民族大学一般科研项目(2016skky03)
北方民族大学国家自然科学基金前期培育项目(2013QZP05)
NSFC-云南联合基金项目(U1202262)
国家自然科学基金项目(31660195)
关键词
印度块菌
云南松
菌根
细菌
高通量测序
培养
根际土壤
Tuber indicum
Pinus yunnanensis
mycorrhizae
bacteria
high-throughput sequencing
culture
mycorrhizal soil
