摘要
目的:探讨同时提取西洋参药材中总多糖和总皂苷的最佳工艺。方法:以西洋参药材中总多糖和总皂苷含量为考察指标,选择乙醇浓度、提取时间、料液比以及提取次数为考察因素,用单因素试验确定因素水平;再以乙醇浓度、料液比以及提取次数为考察因素,用正交试验对最佳工艺提取进行优化。结果:最佳提取工艺条件为:乙醇浓度40%,料液比1∶12,提取次数3次。结论:本研究中所优选的工艺简便、快捷、稳定,可用于同时提取西洋参药材中总多糖和总皂苷。
Objective： To optimize the extraction method for American ginseng. Methods： Based on the contents of the total polysaccharides and saponins in American ginseng,the ethanol concentration,extraction duration,solid-liquid ratio and extraction time were selected to determine the factors and levels by single-factor test. And then 3 factors further considered were the concentration of ethanol,solid-liquid ratio and extraction time. Results： The optimum extraction process was adding 12-fold amount of 40% ethanol,and extracting for 3 times. Conclusion： The optimized extraction process is simple,quick and stable,and can be used to extract the total polysaccharide and saponins from American gingeng.
作者
孙婷婷
张红
李晔
刘洋
王春柳
田欢
刘瑜
Sun Tingting;Zhang Hong;Li Ye;Liu Yang;Wang Chunliu;Tian Huan;Liu Yu(Shaanxi Academy of Traditional Chinese Medicine Institute,Xi＇an 700700,China;Institute of International Trade ＆ Commerce)
出处
《中国药师》
CAS
2018年第10期1734-1737,共4页
China Pharmacist
基金
西安市科技计划项目[编号:2017122SF/YX016(10)]
关键词
西洋参
总多糖
总皂苷
提取工艺
正交试验
American ginseng
Total polysaccharides
Saponins
Extraction technology
Orthogonal Test
