摘要
本文研究烟碱、辛伐他汀、N-甲基吗啉、异烟肼、β-紫罗兰酮5种抑制剂对法夫酵母合成虾青素过程中各中间类胡萝卜素及虾青素含量的影响,以及乙醇对抑制效果的影响。通过高效液相色谱检测发现,5种抑制剂对JMU-VDL668和JMU-MVP14合成类胡萝卜均有抑制作用。其中,法夫酵母JMU-VDL668含有95%左右的虾青素,添加烟碱和异烟肼后β-隐黄质比例明显提升。添加辛伐他汀后,JMU-MVP14虾青素合成途径中的中间类胡萝卜素基本转化为虾青素,虾青素的比例从82.37%提至98.64%。另外,研究发现乙醇对辛伐他汀、N-甲基吗啉引起的代谢抑制有补救作用,而与烟碱、β-紫罗兰酮组合时有叠加的抑制效应。
In this study, five specific inhibitors of the key enzyme in astaxanthin biosynthesis pathway were selected to regulate fermentation process of Phaffia rhodozyma, which contained nicotine, isoniazid, simvastatin, N-methylmorpholine, and β-ionone. Besides, the role of ethanol on the inhibition effect was also studied. HPLC analysis revealed that both JMU-VDL668 and JMU-MVP14 can be affected by these inhibitiors. Among these, JMU-VDL668 contained about95% astaxanthin. The addition of nicotine and isoniazid enhanced the intracellular content of β-cryptoxanthin in Phaffia rhodozyma; likewise, the intermediate carotenoids in astaxanthin biosynthesis pathway of Phaffia rhodozyma JMU-MVP14 were totally converted to astaxanthin after adding simvastatin, and the proportion of astaxanthin was increased from82.37% to 98.64%. The combined addition of inhibitor and ethanolin into the culture medium was studied and the results revealed that ethanol showed a remedial effect on the metabolic inhibition induced by simvastatin and N-methylmorpholine, while ethanol combination with nicotine or β-ionone showed superimposed inhibitory effects.
作者
张枫
沈宁燕
吴昌正
肖琼
倪辉
李利君
肖安风
Zhang Feng;Shen Ningyan;Wu Changzheng;Xiao Qiong;Ni Hui;Li Lijun;Xiao Anfeng(College of Food and Biological Engineering,Jimei University,Xiamen 361021,Fujian;Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province,Xiamen 361021,Fujian;Fujian Provincial Engineering Technology Research Center of Marine Functional Food,Xiamen 361021,Fujian;Xiamen Key Laboratory of Marine Functional Food,Xiamen 361021,Fujian)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2018年第9期76-89,共14页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(31501448)
厦门南方海洋研究中心项目(14CZP035HJ09)
关键词
法夫酵母
虾青素
抑制剂
代谢调控
发酵
Phaffia rhodozyma
astaxanthin
inhibitors
metabolic regulation
fermentation
