摘要
目的:使用穴位贴敷及穴位注射乌体林斯治疗慢性持续期OVA哮喘大鼠并探讨其作用机制。方法:卵蛋白(OVA)致敏并连续激发制作哮喘Wistar大鼠模型,大鼠按随机数字表法分为正常组、哮喘组、贴注组、布地奈德雾化组、联合组5组,治疗1个月后ELISA法测定血清中Ig E,放射免疫法测定肺组织中IL-4、IL-5、ET-1,免疫组化法测定肺组织NF-κB表达,并分析NF-κB的IOD值。结果:模型组血清Ig E,肺组织IL-4、IL-5、ET-1水平,肺组织NF-κB/p65活化情况、IOD值均高于正常组,各治疗组以上指标均低于模型组,联合组较2单独治疗组降低最明显。结论:穴位贴敷联合穴位注射可能通过两方面作用抑制NF-κB的活化,进而抑制下游细胞因子IL-4、IL-5的活性来抑制哮喘炎症的发生,降低ET-1的产生来降低气道高反应性。
Objective： To study the effect of acupoint application combined with acupoint injection of Utlins on OVA induced chronic asthma duration rats. Methods： The model of asthma was established by Ovalbumin. The rats were randomly divided into 5 groups： Normal group,Asthma group,Point application and acupoint injection group,Budesonide atomization group,Combined curative group. Aftar 1 month treatment,the concentration of Ig E in serum was measured through ELISA.The concentration of IL-4、IL-5 and ET-1 in lung tissue were measured through radioimmunoassay. The expression of NF-κBp65 in lung tissue were measured by immunohistochemistry respectively,the relative expression of NF-κBp65 was calculated by the computer image analysis software. Results： The level of Ig E,IL-4,IL-5,ET-1,IOD was higher and the expression of the NF-κBp65 was more active than the normal group. those indicators of the treatment groups were lower than the model group. The improvement was most obvious of combined curative group among three threatment groups. Results：The mechanism of point application and acupoint injection on asthma may be the fouowing aspects： To reduce the inflammatory cytokine of Th2 by inhibiting the activation of NF-κB; To reduce the hyperresponsiveness of the airway by inhibiting the level of ET-1.
作者
梁志娟
耿立梅
LIANG Zhi-juan;GENG Li-mei(Handan Central Hospital,Hebei,Handan 056000,China;Hebei Provincial Hospital of traditional Chinese Medicine,Shijiazhuang 050000,China)
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2018年第9期1273-1275,1321,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
河北省科技厅自然科学基金资助项目(14277798D)-综合外治法对哮喘慢性持续期免疫功能的影响及作用机制研究
