摘要
目的:探讨音乐电针和脉冲电针对快速老龄化(SAMP8)小鼠行为学和海马星形胶质细胞的影响,对比观察其抗痴呆的作用机制。方法:8月龄雄性SAMP8小鼠共30只,随机分为模型组,音乐电针组,脉冲电针组(n=10),以同源的正常老化(SAMR1)小鼠为空白组(n=10),选取"百会"、"印堂"、"人中",每天针刺干预1次,共15天。采用Morris水迷宫观察各组小鼠学习记忆能力变化,运用免疫组化法观察海马GFAP的表达,Western blot检测海马GFAP的蛋白水平。结果:Morris水迷宫提示:与空白组相比,模型组的逃避潜伏期延长(P <0.01),空间探索实验游泳距离增长(P <0.01);与模型组相比,音乐电针组和脉冲电针组逃避潜伏期缩短(P <0.01,P <0.05),游泳路程缩短(均P <0.05)。免疫组化结果:与空白组相比,模型组海马CA1区GFAP平均光密度明显增多(P <0.01);与模型组相比,音乐电针组和脉冲电针组海马CA1区GFAP平均光密度均明显降低(均P <0.01),且音乐电针组GFAP平均光密度低于脉冲电针组(P <0.05)。Western blot检测:与空白组相比,模型组海马GFAP蛋白表达明显增多(P <0.01);与模型组相比,音乐电针组和脉冲电针组GFAP蛋白表达有明显变浅和下降趋势(均P <0.05);音乐电针组GFAP蛋白表达明显低于脉冲电针组(P <0.05)。结论:音乐电针和脉冲电针均可改善SAMP8小鼠学习记忆能力,降低GFAP的表达,可能与其减轻神经炎性反应、改善凋亡,最终达到保护神经元的机制有关,且音乐电针有优于脉冲电针的趋势。
Objective： To observe the effects of music electro-acupuncture and pulsed electro-acupuncture on locomotor avtivity and hippocampal astrocytes in fast-aging （SAMP8） mice, and to compare the anti-dementia mechanism. Methods： Thirty 8-month-old male SAMP8 mice were randomly divided into model group（group M）, music electro- acupuncture group（group MA）, pulsed electro-acupuncture group（group EA）（n=lO）, with homologous normal aging SAMR1 mice as control group（group C）（n=10）. Acupuncture stimulation was applied to ＂Baihui＂ （GV20）, ＂Yintang＂ （GV29） and ＂Renzhong＂（DU26） for 20 rain per day for 15 days. The Morris water maze was used to assess learning- memorize ability. Immunohistochemical DAB staining was used to observe GFAP exptession in hippocampus. Detect GFAP protein levels by Western blot in hippocampus. Results： The Morris water maze test showed ： Compared with group C, the escape latency were increased （P 〈 0.01）, swimming distance were increased in space exploration in group M （P 〈 0.01）. Compared with group M, the escape latency were reduced in group MA and EA （P 〈 0.01, P 〈 0.05） and the swimming distance were shortened （all P 〈 0.05）. The immunohistochemical DAB staining showed： Compared with group C, the average optical density of GFAP in the hippocampal CA I area of group M increased significantly （P 〈 0.01）; the optical density of GFAP in CA1 area of hippocampus in both groups MA and EA was significantly decreased （all P 〈 0.01）, while the optical density of GFAP in MA group was lower than that of EA group （P 〈 0.05）. Western blot test showed： Compared with group C, the expression of GFAP protein obviously increased （P 〈 0.01）. Compared with group M, the expression of GFAP protein obviously decreased and had a downregulation tendency in group MA and EA （all P 〈 0.05）, while the expression of GFAP protein decreased in MA group compare with EA group （P 〈 0.05）. Conclusion： Both music electro-acupuncture and pulsed electro-acupuncture can improve the learning and memory abilities of SAMP8 mice and decrease the expression of GFAP, which may be related to its mechanism of reducing neuroinflammatory reaction, improving apoptosis and eventually protecting neurons, and music electro-acupuncture has a better tendency than pulsed electro-acupuncture.
作者
邵淑君
唐银杉
曹瑾
许安萍
田朝阳
郭郁
向杜炼
李志刚
邬继红
Shao Shujun;Tang Yinshan;Cao Jin;Xu Anping;Tian Zhaoyang;Guo Yu;Xiang Dulian;Li Zhigang;Wu Jihong(School of Acu-moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 10029,China;Second Affiliated Hospital of Zhejiang University School of Traditional Chinese Medicine Rehabilitation,Hangzhou 310000,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2018年第6期953-959,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委青年基金项目(81603678):督脉电针治疗AD双转基因小鼠不同脑区神经元凋亡作用机制的研究
负责人:唐银杉
国家自然科学基金委青年基金项目(81503654):逆针灸对老年性痴呆小鼠"抗衰老基因"Klotho的调控
负责人:许安萍
关键词
阿尔茨海默病
音乐电针
脉冲电针
胶质纤维酸性蛋白
星形胶质细胞
Alzheimer＇s disease
music electro-acupuncture
pulsed electro-acupuncture
glial fibrillary acidic protein
astrocytes
