摘要
目的探讨DNA-微阵列芯片法检验结核与非结核分枝杆菌的临床应用价值。方法选择2016年12月—2018年3月该院痰结核培养或结核分杆菌痰涂片阳性标本108份。108份标本均采用改良罗氏培养法、DNA-微阵列芯片法检验,对二者检验结果不一致的标本进行测序检验,并将结果作为检验的金标准。评价改良罗氏法、DNA-微阵列芯片法对结核与非结核分枝杆菌的鉴别效果。结果改良罗氏培养法对结核分枝杆菌与非结核分枝杆菌检验的准确率为96.15%、97.56%,与DNA-微阵列芯片92.31%、96.34%对比差异无统计学意义(χ~2=0.253、0.265,P>0.05)。结论 DNA-微阵列芯片法在结核与非结核分枝杆菌检验中具有较高的准确率,且操作简便、快速,临床应用价值显著。
Objective To investigate the clinical value of DNA microarray in the detection of tuberculosis and non-tuberculous mycobacteria. Methods A total of 108 positive samples of sputum tuberculosis or tubercle bacillus sputum smear were collected from December 2016 to March 2018 in the hospital. All 108 specimens were tested by modified Roche culture method and DNA-microarray chip method. Sequencing tests were performed on specimens with inconsistent test results, and the results were used as the gold standard for testing. To evaluate the differential effect of modified Roche method and DNA-microarray chip method on tuberculosis and non-tuberculous mycobacteria. Results The accuracy of the modified Roche culture method for detecting mycobacterium tuberculosis and non-tuberculous mycobacteria was 96.15% and 97.56%. There was no significant difference between the modified Roche culture method and DNA-microarray chips 92.31% and 96.34% （χ^2=0.253, 0.265, P〉0.05）. Conclusion The DNA-microarray chip method has high accuracy in the detection of tuberculosis and non-tuberculous mycobacteria, and the operation is simple and rapid, and the clinical application value is significant.
作者
徐微
XU Wei(Department of Clinical Laboratory,Jilin Province Tuberculosis Hospital,Changchun,Jilin Province,130500 China)
出处
《系统医学》
2018年第15期4-6,共3页
Systems Medicine
