摘要
目的观察电针百会及神庭穴改善大脑中动脉栓塞(MCAO)大鼠学习记忆能力的作用机制。方法选取72只SPF级雄性大鼠,随机分为假手术组、模型组和电针组,每组24只,其中模型组和电针组行左侧MCAO,造模成功后电针组予电针干预,模型组仅固定不干预;假手术组仅切开颈部皮肤,分离颈总、颈内和颈外动脉后直接缝合皮肤。观察大鼠学习记忆能力和海马组织病理形态学改变,计算脑梗死体积,检测β-catenin和GSK-3β蛋白和基因表达水平变化。结果与假手术组干预后和本组造模前比较,模型组和电针组干预后游泳轨迹和逃避潜伏期延长,且电针组干预后游泳轨迹和逃避潜伏期较模型组明显缩短,差异有统计学意义(P<0.05)。假手术组未见脑梗死病灶,电针组干预后脑梗死体积百分比较模型组缩小,差异有统计学意义(P<0.05)。假手术组海马组织细胞形态结构正常;模型组缺血侧海马组织细胞排列紊乱,出现坏死及神经元细胞缩小变形;电针组缺血侧海马组织细胞大部分形态结构正常,少数细胞序列散乱,可见部分神经元缺血。模型组和电针组β-catenin和GSK-3β蛋白和基因的表达水平较假手术组下降,同时电针组较模型组β-catenin和GSK-3β蛋白和基因的表达水平明显上升,差异有统计学意义(P<0.05)。结论电针百会穴、神庭穴通过减轻海马神经元损伤及上调β-catenin与GSK-3β蛋白和基因表达水平起到脑保护作用,提高MCAO大鼠的学习记忆能力。
Objective To observe the mechanism of electroacupuncture （EA） at Baihui and Shenting acupoint in improving learning and memory ability of rats with middle cerebral artery occlusion （MCAO）. Methods A total of 72 specific pathogen-free （SPF） male rats were randomly divided into sham operation group, model group and EA group, with 24 rats in each group. MACO on the left side was established in the model group and the EA group. After successful modeling, the EA group was treated with EA, and the model group was only fixed without intervention. In the sham operation group, the neck skin was cut, and the skin was directly sutured after separation of common carotid artery, internal carotid and external carotid artery. The changes of learning and memory ability of rats and pathological changes of hippocampus in rats were observed, the area of cerebral infarction was calculated, and the expressions of β-catenin and GSK-3 β protein and gene expression were detected. Results Compared with the sham operation group after intervention and the groups before the modeling, the swimming trajectory and escape latency of the model group and EA group were prolonged, which were significantly shorter in EA group than in the model group （ P 〈0.05）. There was no infarction in the sham operation group, and the percentage of cerebral infarction volume in EA group was smaller than that in the model group after EA intervention, and the difference was statistically significant （ P 〈0.05）. The morphological structure of hippocampal cells in the sham operation group was normal, whereas the cells in the ischemic side of the hippocampus in the model group were in disorder, and there was necrosis and shrinkage of neuron cells. Most of the cells in the ischemic side of hippocampal tissue in the EA group were normal, while a small number of cells were scattered, and some neurons were ischemic. The levels of protein and gene expression of beta -catenin and GSK-3 beta in the model group and EA group were lower than those in the sham operation group. In the meantime, the expression level of β-catenin and GSK-3 β protein and gene was significantly up-regulated in EA group, as compared with the model group, and the difference was statistically significant （ P 〈0.05）. Conclusion EA at Baihui and Shenting acupoint plays a protective role in the brain by reducing the damage to hippocampal neurons and upregulating the expression level of β-catenin and GSK-3 β and gene, thereby improving learning and memory ability of MCAO rats.
作者
于雪萍
李安洪
江一静
YU Xu-ping;LI An-hong;JIANG Yi-jing(Sichuan College of Traditional Chinese Medicine,Mianyang,Sichuan 621000,China;the First Department of Acupuncture and Moxibustion,Mianyang Hospital of Traditional Chinese Medicine,Mianyang,Sichuan 621000,China;Rehabilitation Department,Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350001,China)
出处
《临床误诊误治》
2018年第9期103-107,共5页
Clinical Misdiagnosis & Mistherapy
基金
高校自然基金青年重点项目(JZ160443)
关键词
梗死
大脑中动脉
电针
记忆障碍
学习障碍
Infarction
middle cerebral artery
Electroacupuncture
Memory disorders
Learning disorders
