摘要
目的检测mi R-106a-5p在胃癌细胞和胃癌组织中的表达并分析其表达与胃癌患者临床病理特征的关系,并采用生物信息学方法分析其靶基因及其富集的信号通路。方法采用实时荧光定量PCR法检测mi R-106a-5p在正常胃黏膜上皮细胞GES-1和不同分化程度的胃癌细胞AGS(高分化)、MKN-28(中分化)、HGC-27(未分化)、MGC-803(低分化)、BGC-823(低分化)、MKN-45(中分化)及SGC-7901(中分化)和组织(58例胃癌组织及其相应的距离癌灶5 cm以上且经HE染色证实均无癌细胞的癌周组织)中的表达,采用mir WALK网站数据库的预测软件预测其靶基因并选择3个以上软件支持的靶基因,运用DAVID 6.7软件在线富集选择的靶基因参与的信号通路。结果与正常胃黏膜上皮细胞GES-1比较,mi R-106a-5p在不同分化程度的胃癌细胞AGS、SGC-7901、MKN-45、MGC-803、BGC-823及HGC-27中的表达量明显上调(P<0.010或P<0.001),而在MKN-28细胞中未见上调(P>0.050)。与相应的癌旁组织比较,mi R-106a-5p在胃癌组织中的表达量在36例中表达上调(即高表达),在18例中表达下调(即低表达),4例变化不明显。mi R-106a-5p在胃癌组织中表达与淋巴结转移(P=0.003)和侵犯深度(P=0.034)有关,而与胃癌患者的年龄、性别、组织学分化程度、TNM分期、淋巴管侵犯、血管侵犯及Borrmann分型无关(P>0.050)。生物信息学分析结果提示,mi R-106a-5p的靶基因富集存在于与肿瘤相关的31个信号通路中。结论 mi R-106a-5p在胃癌细胞系及胃癌组织中高表达并与淋巴结转移以及浸润深度有关,其有可能作为一具备潜在研究价值的癌基因。
Objective To detect expression of mi R-106 a-5 p in gastric cancer cells and gastric cancer tissue,and to analyze relationship between it＇s expression with clinicopathologic characteristics,and in addition,to analyze its target genes and enriched pathway with bioinformatics method.Methods The expressions of mi R-106 a-5 p in the different differentiation of gastric cancer cells AGS（well differentiation）,MKN-28（middle differentiation）,HGC-27（undifferentiation）,MGC-803（low differentiation）,BGC-823（low differentiation）,MKN-45（middle differentiation） and SGC-7901（middle differentiation）,the normal gastric mucosal epithelial cells GES-1,and the gastric cancer tissue and the corresponding adjacent tissue were detected by the real-time fluorescent quantitative PCR.Furthermore,the target genes of mi R-106 a-5 p were predicted by using more than three softwares affiliated to mir WALK web database and the signal pathways of target genes were enriched by DAVID 6.7 software.Results The expressions of mi R-106 a-5 p in the different differentiation degree of the gastric cancer cells（AGS,SGC-7901,MKN-45,MGC-803,BGC-823,and HGC-27） were up-regulated except the MKN-28 cell line as compared with the normal gastric mucosa cell line GES-1（P0.010 or P0.001）,and the expression of mi R-106 a-5 p in the gastric cancer tissue was also up-regulated as compared with the corresponding adjacent tissue,the expression of mi R-106 a-5 p in the gastric cancer tissue was associated with the lymph node metastasis or the invasion depth.The results of the bioinformatics analysis showed that the target genes of mi R-106 a-5 p were enriched in the multiple signaling pathways associated with the cancer.Conclusion mi R-106 a-5 p is a molecular marker of high expression in gastric cancer and a potential cancer gene associated with lymph node metastasis and invasion depth.
作者
康博雄
李海龙
陈彻
马焌峰
樊勇
王琛
KANG Boxiong1, LI Hailong2, CHEN Che2, MA Junfeng1, FAN Yong1, WANG Chen1(1. Department of General Surgery, The Second Hospital of Lanzhou University, Lanzhou 730030, P. R. China; 2. Department of Clinical Testing Teaching and Basic Research of Clinical Laboratory, School of Clinical Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, P. R. Chin)
出处
《中国普外基础与临床杂志》
CAS
2018年第8期923-928,共6页
Chinese Journal of Bases and Clinics In General Surgery
基金
甘肃省高等学校科研项目(甘财教[2014]63-15)
