摘要
目的:探讨血液标本存放时间和温度对全血细胞分析的影响。方法:收集40例全血标本,每1例标本分为两份,分别于室温和4℃条件下存放,每份标本在0 h(即时检测)、2 h、4 h、8 h、12 h、24 h应用迈瑞5180全自动血细胞分析仪进行检测,并进行统计学分析。结果:与即时检测比较,在室温条件下存放标本,血小板平均体积(MPV)在4 h后、红细胞平均体积(MCV)在12 h后检测结果增加,差异有统计学意义(P﹤0.05);4℃存放标本,MPV在24 h后检测结果增加,差异有统计学意义(P﹤0.05)。相同时间室温存放与4℃冰箱存放标本检测结果相比,红细胞平均血红蛋白浓度(MCHC)在8 h后结果降低,MCV在12 h后结果增加,差异有统计学意义(P﹤0.05)。结论:在24 h内,存放时间和条件对全血细胞分析结果均有一定影响,全血细胞分析应在短时间内尽快完成,如不能及时送检,应保存在4℃条件下。
Objective： To investigate the influence of blood specimens storage time and temperature on the blood cell analysis.Methods： Whole blood specimens of 40 cases stored at room temperature and stored at 4℃ respectively,and each specimen was divided into two parts. The specimens were detected by Mindray 5180 automated hematology analyzer for 0 h（ instant test）,2 h,4 h,8 h,12 h and 24 h and analyzed statistically. Results： Compared with immediate test results,mean platelet volume（ MPV） after 4 h and mean corpuscular volume（ MCV） after 12 h showed significant differences at room temperature（ P〈0. 05）. Specimens at 4℃ were significant difference only after 24 h compared with instant test results（ P〈0. 05）. Compared with specimens at 4 ℃ for the same time,mean corpuscular hemoglobin concentration（ MCHC） results decreased after 8 h and MCV results increased after 12 h at room temperature showed significant difference（ P〈0. 05）. Conclusion： Within 24 h,storage time and conditions have a certain impact on the results of whole blood cell analysis. The blood cell analysis should be completed as soon as possible in a short time,if not timely submission,it should be stored at 4℃ condition.
作者
周后蓉
田大斌
张金花
ZHOU Hou-rong;TIAN Da-bin;ZHANG Jin-hua(Department of Nuclear Medicine,Chengdu Military General Hospital of P.L.A,Chengdu 610083;The School Hospital of Southwest Petroleum University;Department of Inspection,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan,Chi-na)
出处
《川北医学院学报》
CAS
2018年第4期551-553,共3页
Journal of North Sichuan Medical College
基金
四川省卫生厅课题(100134)
关键词
血液标本
存放时间
存放温度
全血细胞分析
Blood specimens
Storage time
Storage temperature
Blood cell analysis
