摘要
为了筛选适宜于甜菜品种纯度鉴定的DAMD引物,笔者利用12个不同的甜菜品种分别对26条DAMD引物进行扩增,并分别采用6%聚丙烯酰胺凝胶电泳和2%的琼脂糖凝胶电泳对扩增产物进行检测。结果表明,从26条DAMD引物中筛选出16条扩增清晰的引物,这16条引物共扩增出139条带,其中多态性条带为122条,多态性百分比为87.7%,这16条引物可以作为甜菜品种纯度和真实性鉴定的核心引物,同时发现聚丙烯酰胺凝胶电泳与琼脂糖凝胶电泳的检测结果差异不大,而琼脂糖凝胶具有制作方便、检测简单以及不使用任何有毒药剂等优点,推荐甜菜DAMD扩增产物的检测使用琼脂糖凝胶电泳。
To screen the optimal DAMD primer to identify the purity of different sugar beet varieties, theauthors conducted amplification in 12 varieties with 26 DAMD primers and identified the bands of theseamplification products with 6% SDS-PAGE and 2% agarose gel electrophoresis. The results indicated that： 16 of 26 primers were able to amplify clear products, 139 bands were obtained with these 16 primers, of which122 were of polymorphism and the percentage of polymorphic loci was 87.7%. These 16 primers could be usedas core primers for the identification of purity and authenticity of sugar beet varieties. The authors also foundthat the results of SDS-PAGE and agarose gel electrophoresis had little difference. In conclusion, agarose gelis easy to make, simple for detection and not suitable for any toxic agent, so the detection of beet DAMDamplification product with agarose gel electrophoresis is highly recommended.
作者
孙燕红
马龙彪
兴旺
吴则东
Sun Yanhong;Ma Longbiao;Xing Wang;Wu Zedong(Key Laboratory of Sugar Beet Genetic Breeding/Heilongjiang University,Harbin 150080;Sugar Beet Research Institute,Chinese Academy of Agricultural Sciences/ Crop Academy of Heilongjiang University,Harbin 150080;Key Laboratory of North Sugar Crop Resource and Utilization,Chinese Academy of Agricultural Sciences,Harbin 150080)
出处
《中国农学通报》
2018年第16期42-45,共4页
Chinese Agricultural Science Bulletin
基金
国家糖料现代农业产业技术体系甜菜高品质品种改良岗位专项基金(cars-170111)
农业部物种资源保护费项目"甜菜种质资源收集鉴定编目繁种保存与利用"(1120162130135252026)
科技部
财政部国家科技基础条件平台项目"国家甜菜种质资源平台"(NICGR2017-17)
关键词
甜菜
DAMD
引物筛选
琼脂糖电泳
beet
direct amplification of minisatellite DNA
primer screening
agarose gel electrophoresis
