摘要
为获得生防木霉,用稀释平板法从益母草(Leonurus japonicus)根际土中分离到木霉Lj,经形态及分子鉴定,确定Lj为毛簇木霉(Trichoderma velutinum)。平板对峙培养时,Lj对杨叶枯病菌(Alternaria alternata)、尖孢镰刀菌(Fusarium oxysporum)、杨树烂皮病菌(Cytospora chrysosperma)菌丝生长抑制率为28.4%~60.9%。将Lj固体发酵所产孢子稀释灌土后播种小白菜(Brassica chinensis),孢子浓度为0、109、108、107、106个·mL^(-1),检测时间为播种后15、30、45 d。相比同一时间点对照,处理组叶绿素质量分数明显较高,并随Lj孢子浓度下降而增加,最大值出现在106个·mL^(-1)组。处理组小白菜根长、叶长、鲜质量在15 d和30 d显著高于对照。叶过氧化物酶活性在15d时与对照无显著差异,30 d和45 d时大部分处理组显著高于对照,分别在109、106个·mL^(-1)组最高。Lj菌株具有拮抗真菌和促进小白菜生长的生防功能。
In order to obtain bio-control Trichoderma strain, dilution-plate method was used to isolate Trichoderma from Leonurus japonicus rhizosphere soil, and Lj was isolated. It was identified as T. velutinum by morphology and molecular methods. Moreover, it showed 28.4%-60.9% inhibition ratio on mycelia growth of Alternaria alternata, Fusarium oxysporum and Cytospora chrysosperma on confrontation plates. The spores from Lj solid fermentation were diluted to irrigate the soil, and the contents were 0, 10 9, 10 8, 10 7, and 10 6 ind·mL^-1 . Then the Brassica chinensis was seeded. Its indexes were tested at 15, 30 and 45 d. The chlorophyll mass fractions of leaves in treatment groups were obviously higher than those of the control at the same time point, and they increased with the decrease of Lj spores concentration. The highest value appeared in 10 6 ind·mL^-1 group. The root length, leaf length and fresh weight of plants in treatment group were significantly higher than those of the control group at 15 and 30 d. Leaves peroxidase activity had no significant difference from the control at 15 d. When 30 and 45 d, most of the treatments were significantly higher than those of the control. The maximum activity values were found in 10 9 ind·mL^-1 group at 30 d, and 10 6 ind·mL^-1 group at 45 d. It was considered that Lj strain can antagonize fungi and promote the growth of pakchoi plants.
作者
潘宣圳
曾晓春
刘伟璐
刘志华
王金杰
范海娟
Pan Xuanzhen;Zeng Xiaochun;Liu Weilu;Liu Zhihua;Wang Jinjie;Fan Haijuan(Northeast Forestry University,150040 Harbin,P.R.China)
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2018年第7期73-78,共6页
Journal of Northeast Forestry University
基金
中央高校基本科研业务费专项资金项目(2572014BA15)
东北林业大学大学生创新训练计划项目(201610225152)
关键词
生物防治
木霉
促生作用
小白菜
Biology control
Trichoderma spp.
Plant growth promotion
Brassica chinensis
