摘要
论文利用展示在毕赤酵母表面的稻米脂肪酶作为催化剂在正己烷中催化合成乙酸乙酯,考察了稻米脂肪酶的酶活、底物摩尔比、反应温度、反应时间和3A分子筛添加量对合成乙酸乙酯反应的影响.结果表明,在15 m L正己烷体系中,n(乙酸/乙醇,摩尔比)为1.2∶1,稻米脂肪酶的酶活为15 750 U/g,发酵时间为4 h,反应温度为40℃,3A分子筛添加质量为8%是合成乙酸乙酯的最佳条件。在此条件下,稻米脂肪酶催化合成乙酸乙酯的转化率达到90.92%。
In this study,the rice lipase（RL） was displayed on the surfaceof Pichia pastoris to catalyze synthesis of ethyl acetate in n-hexane,the influence of some factors such as the enzyme activity of rice lipase,substrate concentration,reaction temperature,reaction time,the amount of 3 A molecular sieve on the synthesis of ethyl acetate was investigated. The study showed that,acetic acid/ethanol（mol/mol） ratio1.2 ∶1,reaction time of 4 h,reaction temperature of 40 ℃,the enzyme activityof rice lipase 15 750 U/g,the adding amount of 3 A molecular sieve8 % in 15 m L n-hexane was the optimum condition. Under the condition,the reaction conversion of rice lipase catalyzed synthesis of ethyl acetate reached 90.92%.
作者
焦玲
谢定
文李
姜博
陈平
郑瑞娜
JIAO Ling;XIE Ding;WEN Li;JIA NG Bo;CHEN Ping;ZHENG Ruina(College of Chemical and Biological Engineering,University of Changsha Science and Technology,Changsha 410114,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2018年第5期542-546,共5页
Journal of Food Science and Biotechnology
基金
湖南省自然科学基金项目(2015JJ2010)
粮油深加工与品质控制湖南省2011协同创新项目
关键词
稻米脂肪酶
合成乙酸乙酯
毕赤酵母
rice lipase
synthesis of ethyl acetate
Pichia pastoris
