摘要
枯草芽孢杆菌、蜡样芽孢杆菌、金黄色葡萄球菌和沙门氏菌是食品中常见的腐败菌。为减少其对食品的污染,研究不同生物抑菌剂对4种腐败菌的MIC、MLC,以及在不同质量浓度、pH和螯合剂作用下的抑菌效果。结果表明:ε-PL对4种腐败菌的MIC均为0.125 g/L,MLC除沙门氏菌为0.2 g/L,其余均为0.125 g/L;Nisin对4种腐败菌的MIC依次分别为0.125、0.25、0.25、0.5g/L;MLC分别为2.5、3.0、2.5、3.0 g/L。CTS对4种菌的MIC除沙门氏菌为0.5 g/L,其余均为0.25g/L,CTS对4种菌的MLC除金黄色葡萄球菌为4.0 g/L,其余均为5.0 g/L。3种生物抑菌剂的抑菌效果随着质量浓度的增大而逐渐增强。ε-PL对革兰氏阳性及阴性菌均具有较好的的抑菌效果,在中性条件下(pH=7)抑菌效果最佳。而Nisin与CTS在酸性条件下(pH=3左右)抑菌活性较高。生物抑菌剂与EDTA复配均有一定的协同增效作用,其中ε-PL与EDTA复配的抑菌效果最佳。
Bacillus subtilis,Bacillus cereus,Staphylococcus aureus and Salmonella are common residual spoilage bacteria in food. In order to reduce their pollution on food,MIC,MLC and antibacterial effects of these bio-inhibitors against these four kinds of spoilage bacteria were studied under different concentrations,pH,and with chelating agents in this paper. The results showed that MIC and MLC of ε-PL on these bacteria were all 0.125 g/L except MLC on Salmonella for 0.2 g/L.MIC of Nisin on these four kinds of bacteria were 0.125、0.25、0.25 g/L and 0.5 g/L respectively,MLC of Nisin were 2.5、3.0、2.5 g/L and 3.0 g/L respectively. MIC of CTS on these bacteria were all0.25 g/L except Salmonella for 0.5 g/L. MLC were all 5.0 g/L except Staphylococcus aureus for 4.0 g/L. The antibacterial effects of bio-inhibitors on these spoilage bacteria were gradually enhanced with increase of concentration. ε-PL had the best antibacterial effects on gram positive and negative bacteria,especially under neutral condition（pH=7）. While Nisin and CTS in acidic condition（pH was 3 or so） had higher antibacterial activity. The bio-inhibitors compounded with EDTA had obvious synergistic antibacterial effects,which was the best in ε-PL and EDTA.
作者
郑瑞生
庄端宁
杨莹
陈健伟
吴晓菲
ZHENG Ruisheng;ZHUANG Duanning;YANG Ying;CHEN Jianwei;WU Xiaofei(College of Oceanology and Food Science,Quanzhou Normal University,Quanzhou 362002,Chin)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2018年第5期509-516,共8页
Journal of Food Science and Biotechnology
基金
福建省自然科学基金项目(2017J01646)
福建省高校优秀学科带头人赴海外访学研修项目(闽教师[2016]44号)
国家级大学生创新创业训练计划项目(201613099012
201613099028)
泉州师范学院第四批学科带头人培养项目(泉师人[2016]4号)
泉州师范学院青年博士预研基金项目(2015QBKJ03)
泉州师范学院科研人员启动经费项目(201407)
关键词
生物抑菌剂
腐败菌
复配
抑菌效果
bio-inhibitors
spoilage bacteria
compound
antibacterial effects
