摘要
目的建立余甘子提取物HPLC特征图谱分析方法,并与药材进行了相关性分析。方法采用ZORBAX SB A_qC_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.2%(体积分数)H_3PO_4溶液进行梯度洗脱;检测波长为273 nm;柱温为30℃;流速为1.0 mL/min。结果 10批余甘子药材共标示出12个特征峰,鉴定出2个特征峰(没食子酸、鞣花酸),相似度为0.828~0.979,提示不同批次药材的质量差异较大。12批余甘子提取物中有10批检出与药材一致的特征峰,标示出6个特征峰,相似度为0.914~1.000;有2批为未知伪品,均未检出相应的特征峰。结论本方法建立的特征图谱准确可靠、重复性好,为评价余甘子提取物及余甘子药材的质量提供了依据。
Objective To establish the characteristic spectrum for Phyllanthus emblica extract by HPLC.Methods HPLC was performed on ZORBAX SB AqC18 column（ 250 mm× 4.6 mm,5 μm）,and methonal-0.2% phosphoric acid solution was employed as a mobile phase by gradient elution. The detection wavelength was 273 nm,the column temperature was 30 ℃,and flow rate was 1.0 m L/min. Results 12 specific peaks were separated in the 10 batches of Phyllanthus emblica, 2 of which were identified, and the similarities were 0.828 - 0.979. The similarities of Phyllanthus emblica extract were 0.914 - 1.000 with 6 specific peaks separated from only 10 batches in the 12 batches,while the other 2 batches were counterfeit.Conclusion The method is accurate and reproducible, which could be used for the quality control of Phyllanthus emblica and Phyllanthus emblica extract.
作者
陈晓丹
张俊仪
林伟斌
黄月纯
张文莉
梁芷韵
魏刚
CHEN Xiaodan;ZHANG Junyi;LIN Weibin;HUANG Yuechun;ZHANG Wenli;LIANG Zhiyun;WEI Gang(Infinitus（China）Company Limited,Jiangmen 529156,China;The First Clinical Medical College of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Guangzhou University of Chinese Medicine,School of Chinese Materia Medica,Guangzhou 510006,China)
出处
《广东药科大学学报》
CAS
2018年第3期282-287,共6页
Journal of Guangdong Pharmaceutical University
关键词
余甘子提取物
余甘子
特征图谱
高效液相色谱法
Phyllanthus emblica extract
Phyllanthus emblica characteristic spectrum
HPLC
