摘要
以废弃茶末为原料,接种黑曲霉,以液态摇瓶的形式进行单宁酶生产的研究。通过单因素实验、Plackett-Burman实验及Box-Behnken响应面实验优化黑曲霉B1401液态发酵产单宁酶的最佳工艺条件。结果表明,诱导物为6%单宁酸+3%茶多酚,碳源为复配碳源,即0.746%可溶性淀粉:0.254%蔗糖,氮源为复配氮源,即0.3%尿素:0.7%硝酸钠,接种量为1%,装液量为90 m L/250 m L,转速为140 r·min^(-1),温度为30℃,发酵时间为72 h,此条件下单宁酶酶活可达104.82 U/g。
Using waste tea residue as raw material and Aspergillus niger as fermentation starter, tannase was produced by liquid -state shake flask fermentation.The optimal conditions were determined by single factor experiments test, Plackett-Burman test and Box-Behnken response surface method.Results showed that the optimum conditions for the liquid-state fermentation by Aspergillus niger were：6% tannic acid ：3% tea polyphenols as mixed inducer,O.746% soluble starch ：0.254% sucrose as mixed carbon source,0.3% urea ：0.7% sodium nitrate as mixed nitrogen source,inoculum size 1% ,medium contents 90 mL/250 mL, rotating speeds 140 r·min^-1,temperature 30 ℃ ,the fermentation time for 72 h,the tannase activity reached 104.82 U/g.
作者
胡娜
吴鑫颖
邱树毅
王艳
唐佳代
HU Na1,2, WU Xin-ying1,2, QIU Shu-yi1,2, WANG Yan1,2 , TANG Jia-dai1,2(1.Guizhou Key Lab of Fermentation Engineering and Biological Pharmacy, Guiyang 550025, China; 2.School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, Chin)
出处
《食品工业科技》
CAS
CSCD
北大核心
2018年第15期205-212,共8页
Science and Technology of Food Industry
关键词
黑曲霉
废弃茶末
发酵
单宁酶
响应面法
Aspergillus niger
waste tea residue
fermentation
tannase
response surface method
