摘要
目的研究雷公藤红素通过细胞内活性氧(Reactive oxidative species,ROS)诱导人卵巢癌SKOV3细胞凋亡的作用机制。方法通过MTT比色法检测雷公藤红素对人卵巢癌SKOV3细胞增殖的抑制作用;通过Hoechst 33258染色荧光显微镜观察SKOV3细胞凋亡的形态学变化;通过流式细胞术检测SKOV3细胞凋亡及ROS水平;Western blot法检测凋亡相关蛋白bcl-2、cleaved caspase-3、cleaved caspase-9蛋白的表达。结果雷公藤红素对卵巢癌SKOV3细胞具有明显的增殖抑制作用,且呈时间/剂量依赖关系(P<0.05);雷公藤红素可显著增加细胞内ROS水平,诱导卵巢癌SKOV3细胞凋亡,并上调cleaved caspase-3、cleaved caspase-9蛋白的表达,下调抗凋亡蛋白bcl-2的表达(P<0.05)。结论雷公藤红素可通过提高细胞内ROS水平,诱导人卵巢癌SKOV3细胞凋亡,为卵巢癌治疗及预后方面的研究提供了新思路。
Objective To show the evidence of apoptosis in SKOV3 cells induced by celastrol via alteration of ROS level. Methods The proliferation of SKOV3 cells was determined by MTT staining method;the intracellular ROS levels were detected by flow cytometry;Hoechst 33258 staining and flow cytometry were used to detect the apoptosis of cells;and expression levels of apoptotic proteins were determined by Western Blot method. Results Celastrol had obvious proliferation inhibitory effect on ovarian cancer SKOV3 cells, and showed a time/dose dependent relationship. Celastrol induced significant apoptosis in ovarian cancer SKOV3 cells, which significantly increased intracellular ROS levels,up-regulated the levels of cleaved caspase-3 and cleaved caspase-9,and down-regulated the expression of anti-apoptotic protein bcl-2 in SKOV3 cells. Conclusion Celastrol induced apoptosis in human ovarian cancer SKOV3 cells by increasing intracellular ROS levels.
作者
何冰玉
张亚南
苏荣健
齐凤杰
张蕴莉
HE Bingyu1, ZHANG Yanan1, SU Rongjian1, QI Fengjie2, ZHANG Yunli2(1. Jinzhou Medical University, Jinzhou 121000 Liaoning, China; 2. The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000 Liaoning, Chin)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2018年第4期443-448,共6页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金(81172048)
