摘要
目的:建立一种测定血管紧张素氨基酸组成的柱前衍生高效液相色谱法。方法:血管紧张素经酸水解产生的游离氨基酸,用AQC衍生后,以醋酸钠溶液和乙腈作为流动相,流速为1.0 ml/min,经AccQ·Tag色谱柱梯度洗脱,于波长254 nm处测定。通过外标法计算样品中氨基酸组成。结果:His、Arg、Pro、Tyr、Val、Phe在浓度范围0.02~1.00 mmol/ml内与峰面积呈良好的线性关系,Asp在0.02~0.75 mmol/ml浓度范围内与峰面积呈良好的线性关系。结论:本实验建立的分析方法分离效果较好,可用于血管紧张素氨基酸组成测定和肽含量测定。
Objective： To establish an HPLC method with precolumn derivation to determine amino acid composition and peptide content of angiotensin amide. Methods： The free amino acids were derivatized with AQC after angiotensin amide was hydrolyzed by hydrochloric acid. The derivatives were eluted by gradient solutions composed of sodium acetate solution and acetonitrile on HPLC at flow rate of 1.0 ml/min and detective wavelength of 254 nm. The contents of amino acids in angiotensin amide were calculated based on external standard. Results： The standard curves were linear over the concentration range of 0.02-1.00 mmol/ml for His, Arg, Pro, Tyr, Val, and 0.02-0.75 mmol/ml for Asp. Conclusion： The method is suitable for the analysis of amino acid composition and peptide content of angiotensin amide.
作者
丁金国
李晓倩
黄臻辉
DING Jinguo;LI Xiaoqian;HUANG Zhenhui(SPH NO.1 Biochemical ＆ Pharmaceutical Co.,Ltd.,Shanghai 200240,China)
出处
《上海医药》
CAS
2018年第11期98-102,共5页
Shanghai Medical & Pharmaceutical Journal
关键词
血管紧张素
氨基酸组成
肽含量HPLC
angiotensin amide
amino acid composition
peptide content
HPLC
