摘要
目的探讨不同浓度无机三氧化矿物凝聚体(mineral trioxide aggregate,MTA)对根尖乳头干细胞(stem cells from apical papilla,SCAP)增殖及分化能力的影响及促进其向成牙本质细胞分化的潜力。方法向SCAP分别加入不同浓度MTA培养液,配制成浓度为0.01、0.02、0.1、0.2、1、2、10、20 mg/m L的MTA实验组;设不含MTA而含有15%胎牛血清的α-MEM培养液为对照组,CCK-8法测定1、3、5、7 d的细胞数,观察不同浓度MTA对SCAP增殖的影响,使用Real-time PCR检测分化相关基因牙本质涎蛋白(dentinsialoprotein,DSPP)、Runt相关转录因子(runt-related transcription factor 2,Runx2)表达的变化。结果培养1 d时,各实验组的MTA实验组对SCAP的体外增殖的促进作用与对照组相比差异均无统计学意义(P>0.05);而在培养3、5及7 d时,0.01mg/m L组的体外增殖作用比对照组高,差异有统计学意义(P<0.05),0.02、0.1、0.2、1 mg/m L组与对照组差异均无统计学意义(P>0.05),2、10、20 mg/m L组的体外增殖作用均低于对照组,差异有统计学意义(P<0.05)。Real-time PCR检测显示,经0.01 mg/m L MTA处理7 d的实验组细胞DSPP(t=-11.12,P<0.05)及Runx2(t=-10.62,P<0.05)表达水平高于对照组。结论 0.01 mg/m L浓度组对SCAP的增殖、成牙本质分化及成骨分化有显著促进作用,而高浓度MTA则抑制SCAP的增殖。
Objective To investigate the effect of different concentrations of MTA on the proliferation and differentiation of stem cells fi＇om the apical papilla （SCAP） and the potential of the SCAP to differentiate into odontoblasts. Methods SCAP were cultured in different concentrations of mineral trioxide aggregate （MTA）. MTA experimental group with concentration of 0.01 mg/mL, 0.02 mg/mL, 0.1 mg/mL, 0.2 mg/mL, 1 mg/mL, 2 mg/mL, 10 mg/mL and 20 mg/ mL were prepared. The number of cells at 1 day, 3 days, 5 days and 7 days were measured via a CCK-8 assay to observe the effect of MTA on SCAP proliferation. Real-time PCR was used to detect the gene expression changes. Cells cultured in alpha MEM culture containing 15% FBS without MTA were set as the control group. Results When cultured for 1 d, statistically significant differences in the promotion of in vitro proliferation of SCAP were not observed between each MTA experimental group and the control group （P 〉 0.05）. When cultured for 3 d, 5 d and 7 d, the 0.01 mg/ mL MTA group presented obvious promotion of SCAP proliferation compared with the control group （P 〈 0.05）, whereas the 0.02 mg/mL, 0.1 mg/mL, 0.2 mg/mL, 1 mg/mL groups did not presented differences with the control group （P 〉 0.05）. The in vitro proliferation of the 2 mg/mL, 10 mg/mL and 20 mg/mL groups was lower than that of the control group （P 〈 0.05）. Real-time PCR detection showed that the expression levels of DSPP （t = -11.12, P 〈 0.05） and Runx2 （t = -10.62, P 〈 0.05） in the experimental group treated with 0.01 mg/mL MTA for 7 days were higher than those in the control group. Conclusion The 0.01 mg/mL concentration of MTA significantly promotes the proliferation of SCAP and shows the best ability to induce osteogenic and odontoblast differentiation in the SCAP, whereas high concentrations of MTA inhibited the proliferation of SCAP.
作者
牛巧丽
李一鸣
宋艳艳
李晨曦
赵今
NIU Qiaoli;LI Yiming;SONG Yanyan;LI Chenxi;ZHAO Jin(Department of Endodontics,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;Department of Periodontics ＆ Oral Medicine,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;Department of Prosthodontics,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;Department of Oral and Maxillofacial Surgery,The Head and Neurocenter Tumor Genetics Laboratory,Regenerative Medicine Laboratory University Medical Center Hamburg-Eppendoff（UKE）,Hamburg 20246,Germany)
出处
《口腔疾病防治》
2018年第8期491-495,共5页
Journal of Prevention and Treatment for Stomatological Diseases
基金
新疆维吾尔自治区自然科学基金项目(2016D01C315)
关键词
MTA
根尖乳头干细胞
增殖
分化
基因表达
Mineral trioxide aggregate
Stem cells from apical papilla
Proliferation
Differentiation
Gene expression
