摘要
目的探讨CYP11A1基因rsrs900798位点多态性与老年性骨质疏松性骨折患者骨密度、骨代谢标志物的关联。方法前瞻性选取2016年4月至2018年4月骨科收治的骨质疏松症及骨质疏松性骨折患者共420例,其中220例老年骨质疏松症患者作为对照组,200例老年骨质疏松性骨折患者作为观察组。采用SNa Pshot技术单核苷酸多态性(SNP)分型,测定患者骨密度、骨代谢标志物[血清总骨Ⅰ型前胶原氨基端前肽(Total-PINP)、Ⅰ型胶原羧基端肽β特殊序列(β-CTX)]。观察该基因多态性与患者骨密度、骨代谢标志物的关联。结果观察组与对照组等位基因频率、基因型频率差异均无显著性(P>0.05)。观察组Total-PINP、β-CTX均高于对照组(P<0.05)。观察组GG、GT、TT三种基因型的Total-PINP、β-CTX检测含量差异均无统计学意义(P>0.05)。总髋部、腰椎、股骨大转子、股骨颈、Ward's三角5个部位GG、GT、TT三种基因型骨密度差异均无统计学意义(P>0.05)。结论骨代谢标志物PINP和β-CTX可用来预测骨折危险性,但与CYPll Al基因rs900798位点多态性和其无明显关联,且该位点与骨折患者骨密度无明显关联。
Objective To explore the correlation of Rsrs900798 CYP11A1 gene loci polymorphism and senile osteoporosis fracture in patients with bone mineral density and bone metabolism markers. Methods From April 2016 to April 2018,a total of 420 osteoporosis and osteoporotic fractures patients admitted in our hospital were enrolled in this study,of which 220 cases of senile osteoporosis patients were selected as con-trol group,200 cases of elderly patients with osteoporotic fracture as observation group. SNa Pshot technology single nucleotide polymorphisms（ SNPS） classification was used to determine bone mineral density and bone metabolism in patients with markers（ Total-PINP、β-CTX）. The correlation of gene polymorphisms and bone mineral density,bone metabolic markers was analyzed. Results The allele frequency and genotype frequency in the two groups had no significant difference（ P〉0. 05）. Total-PINP,beta CTX in observation group were higher than those of control group（ P〈0. 05）. Total-PINP,beta CTX of GG,GT,TT three genotypes in observation group had no statistically significant difference（ P〉0. 05）. GG,GT,TT of total hip,lumbar spine,femoral greater trochanter,femoral neck,Ward＇s triangle had no statistically significant difference in the bone mineral density（ P〉0. 05）. Conclusion Bone metabolic markers PINP and beta CTX can be used to predict fracture risk,but its correlation with rs900798 gene loci polymorphism of CYPll Al is not obvious,and the loci is not obviously correlated with bone mineral density of fracture patients.
作者
李智
吴海辉
LI Zhi;WU Hai - hui(Department of Orthopedics,Zhogshan Huspital Qingpu Branch,Shanghai 201700,China.)
出处
《临床和实验医学杂志》
2018年第16期1744-1747,共4页
Journal of Clinical and Experimental Medicine
基金
上海市医学重点专科建设计划(编号:ZK2015B27)
