摘要
研究了黄腐酸对单针藻Monoraphidium sp.FXY-10生长、油脂含量、活性氧(ROS)水平及与油脂合成相关基因表达的影响。采用两阶段培养方法(第一阶段异养培养,第二阶段自养培养),并添加黄腐酸诱导。结果表明:在25 mg/L黄腐酸诱导下,6 d时藻细胞中油脂含量可达54.3%,是对照组的1.12倍,藻细胞内活性氧水平是对照组的1.46倍。此外,油脂合成相关酶基因与油脂合成存在相关性,黄腐酸诱导下,me、acc D、pepc基因表达量分别是对照组的1.32倍(4 d)、1.67倍(4 d)及1.71倍(6 d)。研究表明,黄腐酸提高油脂积累可能与活性氧水平及基因表达有关。
The effects of fulvic acid on the biomass, lipid content, reactive oxygen （ROS） level and the expression of lipid biosynthesis - related genes of Monoraphidium sp. FXY - 10 were studied. Two stage culture method（first heterotrophic and then photoautotrophic） was used and fulvic acid was added to in- duce. The results showed that the lipid content reached 54.3 % in algal ceils treated with 25 mg/L fulvic acid, which was 1.12 times of the control, and the intracellular ROS level was 1.46 times of the control. Moreover, the lipid biosynthesis- related genes was correlated with the lipid synthesis, and the expres- sions of me, accD and pepc were 1.32 times（4 d）, 1.67 times（4 d） and 1.71 times（6 d） of the con- trol. These results demonstrated that fulvic acid significantly increased microalgae lipid accumulation by changing the ROS level and gene expression.
作者
董训赞
车绕琼
赵永腾
李涛
徐军伟
赵鹏
余旭亚
DONG Xunzan;CHE Raoqiong;ZHAO Yongteng;LI Tao;XU Junwei;ZHAO Peng;YU Xuya(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,Chin)
出处
《中国油脂》
CAS
CSCD
北大核心
2018年第7期108-112,共5页
China Oils and Fats
基金
国家自然科学基金项目(21766012
21666012)
