摘要
目的：比较车前子生品和盐炙品水煎液抗大鼠膜性肾病的活性。方法：热回流法提取车前子不同炮制品;选用改良的Border法应用阳离子化牛血清白蛋白（C-BSA）建立大鼠膜性肾病模型,在给药的第6周末检测大鼠24 h尿蛋白定量（UTP）及血清总胆固醇（TC）,甘油三酯（TG）,总蛋白（TP）,尿素氮（BUN）,肌酐（SCr）水平;采用电镜和光镜观察肾脏病理形态学变化,免疫组化法检测各组大鼠肾组织肿瘤坏死因子（TNF）-α,白细胞介素（IL）-1β,转化生长因子（TGF）-β1,核转录因子-κB（NF-κB）p65的表达情况。结果：与空白组比较,模型组大鼠的尿蛋白、肾指数、血清生化指标、肾脏免疫调节因子具有极显著差异（P〈0.01）,肾脏病理形态变化明显;与模型组比较,贝那普利组、盐炙品3个剂量组和生品高剂量组均可显著降低模型大鼠尿蛋白、肾指数（P〈0.05,P〈0.01）,使血清生化指标呈显著差异（P〈0.05,P〈0.01）,病理形态结果显示,大鼠肾脏病变有明显的改善,4种细胞因子表达显著降低（P〈0.01,P〈0.05）;与生品高剂量组比较,贝那普利组和盐炙品高、中剂量组尿蛋白量显著降低（P〈0.05,P〈0.01）,贝那普利组和盐炙品高剂量组肾指数显著降低（P〈0.05）,贝那普利组、盐炙品高、中剂量组血清中BUN,SCr,TC,TG的含量显著低于生品高剂量组（P〈0.05,P〈0.01）,低剂量组仅BUN显著低于生品高剂量组（P〈0.05）,贝那普利组、盐炙品高、中剂量组血清中TP显著升高（P〈0.05）,贝那普利组对肾脏4种免疫调节因子的表达显著降低（P〈0.01）,盐炙品3个剂量组中TNF-α,IL-1β的积分吸光度（IA）显著降低（P〈0.01）,盐炙品高剂量组TGF-β1,NF-κB p65的IA表达显著低于生品高剂量组（P〈0.05,P〈0.01）。结论：车前子盐炙品对膜性肾病大鼠的肾保护作用和对肾脏中免疫调节因子的调节作用均优于生品,可继续深入车前子盐炙品的物质基础和药效机制研究。
Objective： To compare the activities of different processed products of Plantaginis Semen on membranous nephropathy rats. Method： Different processed products of Plantaginis Semen were extracted by heating reflux. The improved Border method was used to establish a model of membranous nephropathy in rats using bovine serum albumin（C-BAS）. The 24 h urinary protein quantitative（UTP）,serum total cholesterol（TC）,triglyceride（TG）,total protein（TP）,blood urea nitrogen（BUN） and serum creatinine（SCr） in the rats in the6 thweekend were tested. The renal pathological morphology changes were observed under electron microscope,and the expressions of tumor necrosis factor（TNF）-α,cell factor of interleukin（IL）-1β,transforming growth factor（TGF）-β1,nuclear factor kappa B（NF-κB） p65 were detected by immunohistochemical methods. Result：Compared with the blank group,the contents of urinary proteins,kidney exponents,biochemistry indexes in serum,immune cytokines in model group were significantly different（P 〈 0. 01）,with obvious pathological changes of kidney. Compared with the model group,the contents of urinary proteins and kidney exponents in benazepril group,three salt-processed groups and high-dose crude Plantaginis Semen group were lower（P 〈 0. 05,P 〈 0. 01）,the changes of biochemistry indexes in serum were obvious（P 〈 0. 05,P 〈 0. 01）,and the pathological changes of kidney were significantly improved in benazepril group,three salt-processed groups and high-dose crude Plantaginis Semen group; the expressions of four immune cell cytokines were lower（P 〈 0. 01,P 〈 0. 05）. Compared with the high-dose crude Plantaginis Semen group,the contents of urinary proteins in benazepril group,high and middle-dose salt-processed groups were lower（P 〈 0. 05,P 〈 0. 01）,the kidney exponents in benazepril group and high-dose salt-processed group were lower（P 〈 0. 05）. BUN,SCr,TC,TG in benazepril group,and high and middle-dose salt-processed groups were lower（P 〈 0. 05,P 〈 0. 01）,only BUN in low-dose salt-processed group was lower（P 〈 0. 05）,TP in benazepril group,high and middle-dose salt-processed groups were significantly increased（P 〈 0. 05）. Expressions of four biochemistry indexes in serum in benazepril group were significantly lower（P 〈 0. 01）. Compared with the high-dose crude Plantaginis Semen group,expressions of TNF-α,IL-1β in salt-processed groups were lower（P 〈 0. 01）,expressions of TGF-β1,NF-κB p65 in high-dose salt-processed group were lower（P 〈 0. 05,P 〈 0. 01）. Conclusion： The renal protection and immune regulating factor of salt-processed Plantaginis Semen were better than those of crude groups in membranous nephropathy rats. The material basis and pharmacology mechanism of salt-processed Plantaginis Semen were studied in further studies based on current results.
作者
赵宏
李洋
柴桂芳
刘颖斐
管勇舟
匡海学
ZHAO Hong;LI Yang;CHAI Gui-fang;LIU Ying-fei;GUAN Yong-zhou;KUANG Hai-xue(College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China;College of Pharmacy, Jiamusi University, Jiamusi 154007, China;Port Hospital of Tianjin, Tianjin 300450, China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第15期125-131,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点基础研究发展计划(973计划)项目(2013CB531801)
中国博士后科学基金面上项目(2015M571454)
黑龙江省博士后科学基金面上项目(LBH-Z14204)
黑龙江省教育厅青年创新人才项目(UNPYSCT-2016095)
佳木斯大学青年创新人才培养计划项目(22Zq201501)
关键词
车前子
不同炮制品
膜性肾病
Border法
肾指数
免疫组化染色
Plantaginis Semen
different processed products
membranous nephropathy
Border method
kidney exponent
immunohistochemical staining
