摘要
目的建立反相高效液相色谱法(high performance liquid chromatography,HPLC)测定保健食品中功效成分腺苷。方法样品中腺苷经85%的甲醇溶液加热回流提取,C18反相柱分离,在波长260 nm处进样检测。结果在5.045~252.250μg/m L范围内具有良好的线性关系,回归方程是Y=27281X-57639,相关系数r=0.9999,检出限为0.5μg/m L(S/N=3),此方法的回收率为91.5%~97.7%,相对标准偏差(relative standard deviation,RSD)为1.5%~2.8%。结论此方法简便快速,准确可靠,可用于各类保健食品中腺苷含量的测定。
Objective To establish a method for determination of adenosine of functional ingredients in health food by reversed phase-high performance liquid chromatography（HPLC）. Method The adenosine from sample was heating refluxed by 85% methanol, and then separated by C18 column and detected at the wavelength of 260 nm. Results There was a good linear relationship in the range of 5.045-252.250 μg/m L. The regression equation was Y=27281 X-57639, with the correlation coefficient（r） of 0.9999, and the limit of detection was 0.5 μg/m L（S/N=3）. The recoveries were 91.5%-97.7%, and relative standard deviations（RSDs） were 1.5%-2.8%. Conclusion This method is simple, rapid, accurate and reliable, which can be used for the determination of adenosine content in all kinds of health food.
作者
孙艳芳
张剑峰
王缘
张春玲
孙海会
SUN Yan-Fang;ZHANG Jian-Feng;WANG Yuan;ZHANG Chun-Ling;SUN Hai-Hui(Heilongjiang Province Center for Disease Control and Prevention, Harbin 150030, China)
出处
《食品安全质量检测学报》
CAS
2018年第10期2475-2479,共5页
Journal of Food Safety and Quality
关键词
腺苷
高效液相色谱法
保健食品
adenosine
high performance liquid chromatography
health food
