摘要
目的：研究泽泻多糖提取物对糖尿病大鼠肝脏糖脂代谢及肝脏脂联素受体α（Adipo R2）,过氧化物酶体增殖物激活受体（PPARα）mRNA和蛋白表达的影响,并探讨其可能的作用机制。方法：采用腹腔注射链脲佐菌素（streptozotocin,STZ）建立糖尿病大鼠模型,并随机分组为正常组、模型组、盐酸吡格列酮组、泽泻多糖高、中、低（400,200,100 mg·kg-1）剂量组,每组8只,给予相应的药物治疗30 d。实验结束后,比较各组大鼠的体质量,肝重,葡萄糖耐受量（OGTT）,空腹血糖值（FBG）,空腹胰岛素水平（FINS）,糖化血清蛋白（GSP）,糖化血红蛋白（HbA1c）,血清总胆固醇（TC）,甘油三脂（TG）,低密度脂蛋白（LDL）,高密度脂蛋白（HDL）及肝脏形态组织学等指标;免疫组化法观察大鼠胰腺胰岛素样生长因子（IGF）水平;实时荧光定量PCR（Real-time PCR）法和蛋白免疫印迹法（Western blot）分别检测肝脏组织中Adipo R2,PPARαmRNA和蛋白表达水平。结果：与模型组比较,泽泻多糖能明显降低糖尿病大鼠的体质量和肝重（P〈0.01）,并能明显降低大鼠FBG,HbA1c,GSP水平（P〈0.05）,明显降低TC,TG,LDL水平（P〈0.01）,升高HDL指标水平（P〈0.01）;升高肝脏Adipo R2,PPARαmRNA水平（P〈0.05）和蛋白表达水平（P〈0.01）。结论：泽泻多糖具有显著调节糖尿病大鼠糖脂代谢的作用,其作用机制与其提高肝脏Adipo R2,PPARαmRNA和蛋白表达水平相关联,并对糖尿病并发症和肝脏组织脂肪积累具有一定的防治作用。
Objective： To study the effect of Alismatis Rhizoma polysaccharide(AP） on glucose-lipid metabolism as well as the mRNA and protein expression of Adipo R2 and PPARα in diabetic rats,and discuss the potential mechanism. Method： Diabetic rats model was induced by intraperitoneal injection of streptozotocin(STZ）,and the diabetic rats were randomly divided into six groups,including normal group,model group,Pioglitazone hydrochloride group,AP high,medium and low(400,200,100 mg·kg-1） dose groups,n = 8 in each group. The drug administration was given for 30 days. The body weight,liver weight,oral glucose tolerance test(OGTT）,fasting blood glucose(FBG）,fasting insulin(FINS）,glycosylated serum protein(GSP）,glycosylated hemoglobin(Hb A1 c）,serum total cholesterol(TC）,triglyceride(TG）,low density lipoprotein(LDL）,high density lipoprotein(HDL） and liver histology index were compared. Immunohistochemical method was used to observe the level of insulin-like growth factors(IGF）. The protein expression levels of Adipo R2 and PPARα in liver tissues of different groups were measured with Western blot,and the mRNA expression levels were measured with Real-time PCR. Result： As compared with the model group,AP could significantly reduce the body weight and liver weight of diabetic rats(P〈0. 01）,as well as the levels of FBG,Hb A1 c and GSP,improve the indexes of TC,TG,LDL and HDL(P〈0. 05,P〈0. 01）,and regulate the mRNA and protein expression levels of Adipo R2 and PPARα in liver tissues(P〈0. 05,P〈0. 01）. Conclusion： AP can significantly adjust the glucolipid metabolism in diabetic rats,and its potential mechanism is related to the regulation of the expression levels of Adipo R2 and PPARα,with certain prevention and treatment effectfor diabetes complications and fatty accumulation in liver tissues.
作者
钱增堃
崔凡
凌云熹
朱文娟
李小勤
茆政
李敏廷
QIAN Zeng-kun;CUI Fan;LING Yun-xi;ZHU Wen-juan;LI Xiao-qin;MAO Zheng;LI Min-ting(The First People＇s Hospital of Wuhu, Wuhu 241000, China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第11期117-125,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家高技术研究发展计划(863计划)项目(2014AA022304)
关键词
泽泻多糖
糖尿病
糖脂代谢
Alismatis Rhizoma polysaccharide
diabetes mellitus
glucose-lipid metabolism
