摘要
为揭示不同海拔梯度下海南凤仙花可培养根际真菌与细菌群落的动态变化,笔者在不同季节采集了高海拔(1 000 m)、中海拔(400 m)、低海拔(190 m)3个梯度的海南凤仙花根际土壤,采用形态结合分子检测技术检测分离培养到的真菌和细菌,并进一步分析微生物群落组成和动态变化规律。结果表明,在3种海拔梯度的生境中,真菌OTU数量、多样性指数、丰富度指数在干季高于湿季;而细菌的OTU数量、多样性指数、丰富度指数则表现为湿季高于干季。根际真菌群落中木霉属、篮状霉属、青霉属、曲霉属等优势种,在高海拔旱季出现的频度最高;而根际细菌群落中芽孢杆菌属、伯克霍尔德氏菌属、类芽孢杆菌属等优势种,在低海拔的湿季出现频度最高。海南凤仙花根际微生物群落的主要驱动因子是干湿季,其次是海拔。海南凤仙花生长的石灰岩地区,根际真菌与细菌对季节与海拔梯度的响应呈相反趋势。
The rhizosphere soils of Impatiens hainanensis in different seasons were collected at different altitudes,high（1 000 m）,medium（400 m） and low（190 m）,and the fungi and bacteria of the collected samples were isolated by using the morphological method and molecular detection technology and cultured for analysis of the microbial community composition and dynamic changes to reveal the dynamic changes of fungi and bacterial communities in the rhizosphere of I.hainanensis at the different altitudes.The results show the operational taxonomic unit（OUT） number,diversity index and richness index of the fungi in the rhizophere in the habitats of the different altitudes were higher in the dry season than in the wet season,while the OTU number,diversity index and abundance index of the bacteria was higher in the wet season than the dry season.Dominant species of Trichoderma,Talaromyces,Penicillium,and Aspergillus in the rhizosphere fungal community were observed most frequently in the high altitude in the dry season and the dominant bacterial species of Bacillus and Burkholderia in the rhizosphere bacterial community were most found at the low altitude in the wet season.The redundancy analysis of fungi and bacterial communities in the rhizosphere indicated the dry and wet season were the main driving force of the rhizosphere microbial community,followed by altitudes.In the limestone area where I.hainanensis grows,rhizosphere fungi and bacteria showed an opposite trend in response to the seasons and altitudinal gradients.
作者
刘宇
韩淑梅
宋希强
丁琼
王鹏
赵莹
LIU Yu;HAN Sumei;SONG Xiqiang;DING Qiong;WANG Peng;ZHAO Ying(Institute of Tropical Agriculture and Forestry/ Research Center for Terrestrial Biodiversity of the South China Sea, Hainan University, Haikou , Hainan 570228, China;Tropical Crops Genetic Resoures Institute, Chinese Academy of Tropical Agricultural Sciences Danzhou , Hainan 571737 , China)
出处
《热带生物学报》
2018年第1期47-53,共7页
Journal of Tropical Biology
基金
海南大学科研启动基金(kygd1625
kyqd1642)
国家自然科学基金(31560229)
海南省研究生创新科研课题(Hys2017-90)
关键词
海南凤仙花
菌根真菌
细菌
植物群落
石灰岩生境
Impαtiens hαinαnensis
mycorrhizal fungi
bacterium
vegetation community
limestone habitat
