摘要
目的建立同时测定川芎-醋香附药对中阿魏酸、洋川芎内酯I、洋川芎内酯H、洋川芎内酯A、Z-藁本内酯、丁烯基苯酞及α-香附酮7种有效成分的高效液相色谱(HPLC)分析方法。方法采用Kromasil-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇(A)-0.1%甲酸水溶液(B)梯度洗脱,柱温25℃,检测波长分别为250 nm和286 nm,流速1.0 m L/min。结果 7种有效成分的线性关系良好;各成分平均回收率分别为99.7%、99.6%、98.9%、101.2%、99.4%、100.3%、99.0%,RSD分别为1.7%、1.5%、1.3%、2.3%、2.2%、1.6%、2.1%;其余方法学考察结果符合要求。结论该方法简便、快速、准确,可用于川芎-醋香附药对中7种有效成分的同步测定,同时可为川芎-醋香附药对全面质量评价和质量控制提供科学依据。
Objective To develop a HPLC method for simultaneous determination of ferulic acid,Senkyunolide I,Senkyunolide H,Senkyunolide A,z-ligustilide,Butylidenephthalide and α-Cyperone in Ligusticum chuanxiong Hort.-Cyperus rotundus L.. Methods The HPLC system consisted of a Kromasil-C18 column（ 4. 6 mm × 250 mm,5 μm）,with the mobile phase of methanol solution（ A）-water solution containing 0. 1% formic acid（ B） for gradient elution. DAD detector was used and the detection wave lengths were 250 nm and286 nm. The flow rate was 1. 0 m L/min and the column temperature was 25 ℃. Results The liner relationships of seven effective components were favorable; the mean rate of recovery of each were 99. 7%,99. 6%,98. 9%,101. 2%,99. 4%,100. 3%,99. 0%,and RSD were 1. 7%,1. 5%,1. 3%,2. 3%,2. 2%,1. 6%,2. 1%; other methodological investigation results followed the requirements. Conclusion This method is simple,rapid and correct,and it can be used for simultaneous determination of seven effective components in Ligusticum chuanxiong Hort.-Cyperus rotundus L.,and meanwhile,provide scientific evidences for overall quality evaluation and control of it.
作者
郭丽
翟永松
王敏
许永崧
龚慕辛
吴莎
GUO Li;ZHAI Yong-song;WANG Min;XU Yong-song;GONG Mu-xin;WU Sha(School of Traditional Chinese Medicine, Capital Medical University, Beijing Key Lab of Traditional Chinese Medicine Collateral Dizease Theory Research, Beijing 100069)
出处
《北京中医药》
2018年第3期225-229,共5页
Beijing Journal of Traditional Chinese Medicine
基金
北京市自然科学基金资助项目(7174281)
国家自然科学基金资助项目(81703722)
首都医科大学校自然科学基金资助项目(17ZR28)
关键词
高效液相色谱
川芎-醋香附
有效成分
质量控制
HPLC
Ligusticum chuanxiong Holt. Cyperus rotundus L.
effective components
quality control
