摘要
为探究不同山茶科植物叶片铝含量的差异性及铝的积累部位,本研究以22种常见山茶科植物叶片为实验材料,用差速离心法进行叶片亚细胞分级,用铝试剂法测定新老叶的叶片、细胞壁、细胞器、细胞可溶性组分铝含量。结果表明:山茶科植物叶片铝含量表现出明显的富集现象,且不同物种富集铝的能力存在显著差异,22种山茶科植物新叶铝含量在1 106.44~11 542.04 mg/kg之间,老叶铝含量在2 147.15~19 364.70 mg/kg之间,其中红皮糙果茶新叶铝含量和老叶铝含量均为最高;老叶铝含量比新叶铝含量高34%~184%;叶片内的铝主要累积在细胞壁,其铝含量占整个叶片的64.83%~95.80%,且铝积累的比例随叶片的成熟而增加;叶片的细胞器和可溶性组分铝含量均较低,其铝含量在不同物种间有显著性差异;叶片总铝含量与细胞壁铝含量、新叶与老叶总铝含量、新叶与老叶细胞壁铝含量均呈极显著正相关关系(p<0.01)。
The purpose of this study was to explore the differences of aluminum content and accumulation site of aluminum in leaves of different Camellia plants. The leaves of 22 kinds of common Camellia plants were selected as experimental materials. Subcellular classification was achieved by differential centrifugation and the aluminum contents of old and tender leaves, cell wall, organelle and soluble component were determined by the method of aluminum reagent. The results showed that the aluminum contents of Camellia plants were concentrated on leaves showing obvious enrichment, and there were significant differences in the ability of accumulation of aluminum among different species. Aluminum contents in tender leaves of the 22 Camellia species were between 1 106.44-11 542.04 mg/kg, while those in old leaves were between 2 147.15-19 364.70 mg/kg. Among the 22 species,Camellia crapnelliana had the highest content of aluminum in tender leaves and old leaves. The aluminum content of old leaves was 34%-184% higher than that in the tender leaves. Aluminum in the leaves was mainly accumulated in the cell wall, which accounted for 64.83% -95.80% of the whole leaves, and the proportion of aluminum accumulation increased with the maturity of leaves. Both of the aluminum content in organelle and soluble component of leaves were low, and there were significant difference among different species. There were positive correlations between the total aluminum content in leaves and that in cell wall of leaves, the total aluminumcontent in tender leaves and old leaves, and between aluminum content in cell wall of tender leaves and old leaves（p〈0.01）.
作者
渠心静
陈雪燕
戴生玉
袁军
Qu xinjing ,Chen Xueyan ,Dai Shengyu, Yuan Jun(Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Education Ministry, Central South University of Forestry and Technology, Changsha, 410004)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第3期997-1006,共10页
Molecular Plant Breeding
基金
国家自然科学基金项目(31400582)资助
关键词
山茶科植物
新叶
老叶
铝含量
亚细胞
Camellia plants, Tender leaf, Old leaf, Aluminum content, Subcellular
