摘要
乙稀应答因子(ethylene-responsive factor,ERF)是乙烯信号通路中的组分之一,通过认别并结合下游功能基因启动子顺式作用元件GCC-box,从而实现对植物发育的调控和逆境胁迫的响应。本研究以甜瓜品种河套蜜瓜为研究材料,从成熟甜瓜果实中克隆得到554 bp的CmERFV-4基因cDNA,基于该cDNA序列,进行了蛋白理化性质、空间结构与系统进化分析。实时荧光定量PCR检测表明,在不同组织检测结果中CmERFV-4基因在子叶中的表达量最高,在不同发育阶段果实检测结果中该基因在授粉后40 d果实中的表达量最高。构建了该基因超表达载体和RNAi载体。
In ethylene signal transduction pathway, ethylene-responsive factors （ERF） is the downstream component which can regulate plant development and response to stress by identifying and connecting with the cis element GCC-box of target gene. In this study, CmERFV-4 gene cDNA, 554 bp, was cloned from ripe fruit of melon cultivar Hetao （Cucumis melo）. Based on the cDNA sequence, we analyzed the physicochemical properties, spatial structure and phylogenetic analysis of CmERFV-4. Real-time quantitative PCR results showed that the level of expression was highest in the cotyledon during the test of different tissues but when the test was in different developing periods, the expression level was the highest in the 40 d fruit. The over-expression vector and RNAi vector of this gene were constructed.
作者
刘昌
关小雨
林燕
张瑾
哈斯阿古拉
Liu Chang, Guan Xiaoyu, Lin Yan, Zhang Jin ,Hasi Agula(Inner Mongolia Key Laboratory of Herbage ＆ Endemic Crop Biotechnology, College of Life Sciences, Inner Mongolia University, Hohhot, 01002)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第5期1390-1395,共6页
Molecular Plant Breeding
基金
高等学校博士学科点专项科研基金资助课题(博导类)(20131501110007)资助
关键词
甜瓜
ERF
乙烯
转录因子
Melon, ERF, Ethylene, Transcription factor
