摘要
为保护植物乳杆菌的活性以增强乳杆菌在动物肠道内的益生功能,以天然发酵玉米青贮饲料中优良植物乳杆菌作为芯材,乳清蛋白和明胶为壁材,利用喷雾干燥法制成微胶囊,并以植物乳杆菌包埋率为响应值,研究壁材配比、壁材添加量、进风温度、进料量4个因素,进行中心组合实验(Box-Behnken),通过响应面分析对喷雾干燥法制备植物乳杆菌微胶囊条件进行优化。结果表明:最优条件为壁材配比(乳清蛋白与明胶质量比)1∶2、壁材添加量22%、进风温度127℃、进料量35%,在此条件下,植物乳杆菌包埋率为62.15%。结论:本研究为应用喷雾干燥法制备植物乳杆菌微胶囊奠定了基础。
In order to protect the activity of Lactobacillus plantarum to enhance the probiotic function of Lactobacillus in animal intestine ,with Lactobacillus plantarum in natural fermented corn silage as the core material and whey protein and gelatin as the wall material, by spray drying technique were used to make the microcapsules.The survival rate of Lactobacillus plantarum was determined as response value,4 factors （i.e., the ratio of the wall, solid content, inlet air temperature, and feed rate）were selected as the response surface to optimize the conditions.The results showed that the optimal conditions were as follows ： 1 ： 2 ratio of wall material （whey protein： gelatin）,22% solid content of wall material, inlet air temperature of 127℃ and feed amount of 35% .Under these conditions, LactobaciUus plantarum embedding rate was 62.15 % .In conclusion, this study laid the foundation for the preparation of Lactobacillus plantarum microcapsules by spray drying method.
作者
罗红霞
李晓红
田文静
张俊
王建
林少华
LUO Hong-xia1, LI Xiao-hong1, TIAN Wen-jing1, ZHANG Jun2 , WANG Jian1, LIN Shao- hua1(1 .Beijing Agricultural Vocational College, Beijing 102442, China 2.Jiangxi Agricultural University, Nanchang 330045, Chin)
出处
《食品工业科技》
CAS
CSCD
北大核心
2018年第7期110-114,121,共6页
Science and Technology of Food Industry
基金
北京农业职业学院技术研发与示范推广基金<优良乳酸菌特性分析筛选及微胶囊化研究>(XY-YF-17-10)
关键词
植物乳杆菌
微胶囊
喷雾干燥
乳清蛋白
明胶
Lactobacillus plantarum
microcapsutes
spray drying
whey protein
gelatin
