摘要
目的建立十色流式方案检测HIV/AIDS患者外周血中T淋巴细胞亚群及活化状态。方法按多色流式配色原则,初步确立抗人CD3、CD4、CD8、CD45RA、CD25、CCR7、CD28、CD38、HLA-DR抗体及7-AAD的配色方案。采用1例HIV/AIDS患者外周血PBMC标本进行最适电压调节、荧光补偿设定和FMO对照;设定条件后,检测5例HIV/AIDS患者的外周血样本。结果建立检测人外周血中T淋巴细胞亚群及活化状态的最优十色流式染色方案,采用此方案检测5例HIV/AIDS患者外周血标本,分析T淋巴细胞各亚群比例及活化程度,发现不同细胞亚群中活化分子HLA-DR、CD38、CD28的表达模式存在显著差异。结论十色流式方案可检测HIV/AIDS患者外周血中T淋巴细胞亚群及其活化状态操作简便,结果可靠。
Objective To establish the ten-color flow cytometry for measuring T cell subpopulations and their activated state in the peripheral blood of HIV/AIDS patients. Methods The monoclonal antibodies targeting cell surface antigen CD3, CD4, CD8, CD45RA, CD25, CCR7, CD28, CD38 and HLA-DR to establish the ten-color panel were used. One peripheral blood PBMC sample from a HIV/AIDS patient was applied for adjustment of voltage, compensation and FMO control. After that peripheral blood samples, of 5 patients with HIV/AIDS were analyzed by ten-color flow cytometry. Result The ten-color flow cytometric staining method was established for the measurement of T cell subpopulations and activation state in human peripheral blood. This method was applied to detect peripheral blood samples from 5 patients with HIV/AIDS and was able to evaluate the immune status of these patients. And then the percentage of T lymphocyte subgroup and the activation level were detected, respectively. The expression patterns of HLA-DR, CD38 and CD28 in different subpopulations were with significant difference. Conclusions The ten-color flow cytometry was a simple and reliable method for the analysis of T cell subpopulations and their activation in HIV/AIDS patients.
作者
宋洋子
孔雅娴
郝禹
姜钰
汪笛
徐玲
种雪静
戴国瑞
赵红心
曾辉
Song Yangzi1, Kong Yaxian1, Hao Yu1, Jiang Yu1, Wang Di2, Xu Ling2, Chong Xuejin2, Dai Guorui2, Zhao Hongxin2, Zeng Hui1(1.Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China;2.Infectious Center, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, Chin)
出处
《中华实验和临床感染病杂志（电子版）》
CAS
2018年第1期28-34,共7页
Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)
基金
北京市医院管理局"青苗"计划专项经费资助(No.QML20161803)
北京市科技新星计划(No.Z171100001117016)
关键词
多色流式
人类免疫缺陷病毒
获得性免疫缺陷综合征
T细胞亚群
活化
Muti-color flow cytometry
Human immunodeficiency virus
Acquired immuno deficiency syndrome
T cell
Activated status
