摘要
目的研究ACTL6A(actin-like protein6A)在乳腺癌及癌旁正常组织中的蛋白表达特点,及其与乳腺癌患者临床病理特征的相关性。方法采用免疫组织化学法检测ACTL6A蛋白在乳腺浸润性导管癌组织(IDC)以及与其对应的癌旁正常组织(ANBT)中的表达,并分析其与患者的年龄、组织学分级、TNM分期、淋巴结转移、雌激素受体(ER)、孕激素受体(PR)、人类表皮生长因子受体2(Her-2)的关系。结果 ACTL6A在IDC和ANBT中存在差异性表达(Hc=27.746,P<0.01),在IDC中的表达率(63.5%)显著高于ANBT(27.0%),差异有统计学意义(x^2=19.881,P<0.01)。ACTL6A蛋白高表达与乳腺癌患者的淋巴结转移、ER表达有关(P<0.05或0.01),但与TNM分期、年龄、组织学分级、PR和Her-2无关(P>0.05)。结论 ACTL6A或可作为乳腺癌早期诊断的分子标志物,其高表达可影响乳腺癌患者的预后。
Objective To investigate the expression of actin-like protein 6A（ACTL6A） in breast cancer and adjacent normal tissues and its correlation with clinicopathological features of breast cancer patients. Methods Immunohistochemical method is used to detect the expression of ACTL6A protein in breast invasive ductal carcinoma（IDC） and corresponding adjacent normal breast tissues（ANBT）. The relationship between the expression and the age, histological grading, TNM staging, lymph node metastasis, estrogen receptor（ER）,progesterone receptor（PR） and human epidermal growth factor receptor 2（Her-2） is analyzed. Results There was significant difference in the expression of ACTL6A protein in IDC and ANBT（X^2=27.746, P〈0.001）. The expression of ACTL6A protein in IDC（63.5%） was significantly higher than that in ANBT（27.0%）, and the difference was statistically significant（X^2=19.881, P〈0.001）. The high expression of ACTL6A was positively correlated to the lymphatic metastasis and ER expression in breast cancer patients（X^2=5.805, P=0.016; X^2=12.746,P〈0.001）;but uncorrelated to the TNM staging, age, histological grading, PR and Her-2. Conclusion ACTL6A may be used as a new molecular marker for the early diagnosis of breast cancer. The high expression of ACTL6A protein may affect the prognosis of breast cancer patients due to the promotion of lymphatic metastasis and inactivation of ER signaling pathway in breast cancer.
作者
陈建丽
陈晓文
张永喜
CHEN Jian-li;CHEN Xiao-wen;ZHANG Yong-xi(The Third Department of Medical Oncology, the Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453000, China;Oncology Center, the Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524000, China)
出处
《广东医科大学学报》
2017年第6期625-627,共3页
Journal of Guangdong Medical University
基金
河南省教育厅人文社会科学研究项目(No.2016-gh-205)
