摘要
目的探讨miR-146a在脊髓损伤炎症反应中作用机制。方法脊髓损伤大鼠模型静脉注射miR-146a抑制剂/激动剂,并检测IRAK1和TRAF6及下游炎症因子表达,利用萤光素酶实验验证miR-146a是否直接作用于IRAK1和TRAF6基因。结果阴性对照组miR-146a表达在脊髓损伤后明显上调(P<0.01)。大鼠IRAK1和TRAF6基因及下游炎症因子表达在静脉注射miR-146a抑制剂后显著增加(P<0.01),而在注射miR-146a激动剂后显著减少(P<0.01)。miR-146a明显抑制含有IRAK1和TRAF6基因3'UTR区荧光素酶载体表达(P<0.01)。结论 miR-146a可靶向下调IRAK1和TRAF6基因表达,在脊髓损伤恢复过程中发挥积极作用。
Objective To investigate the action mechanism of miR-146a on spinal cord injury（SCI）-induced inflammation response. Methods miR-146a inhibitors/mimics were intravenously injected into rat SCI model. Expression of IRAK1,TRAF6 and downstream inflammatory cytokines was detected. The direct effect of miR-146a on IRAKI and TRAF6 genes was testified by luciferase reporter assay. Results miR-146a was over-expressed after SCI in negative control group（P〈0.01）.Expressions of IRAK1, TRAF6 and downstream inflammatory cytokines was increased after injection of miR-146a inhibitors（P〈0.01）,but decreased after injection of miR-146a mimics（P〈0.01）. miR-146a remarkably inhibited expression of luciferase vector incorporating 3＇UTR of IRAK1 and TRAF6. Conclusion miR-146a can downregulate expression of target genes IRAK1 and TRAF6, which plays a positive role in SCI recovery.
作者
周玉兰
闫守泉
江莲英
王家丰
ZHOU Yu-lan;YAN Shou-quan;JIANG Lian-ying;WANG Jia-feng(Clinical Research Center;Stem Cell Research and Clinical Translation Center;Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, Chin)
出处
《广东医科大学学报》
2017年第6期596-600,605,共6页
Journal of Guangdong Medical University
基金
国家自然科学基金(No.81600445)
广东省医学科研基金(No.A2016522)
广东医科大学附属医院博士科研基金(No.528B20150012)
广东医科大学科研基金(No.2XJ14006
2XJ14040)
