摘要
目的研究唐山地区汉族人群载脂蛋白B(apolipoprotein B,Apo B)基因7673C/T和11039G/A多态性分布与脑梗死的关系。方法应用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)检测2016年6-12月本院90例脑梗死患者和同期82例正常对照者Apo B基因7673C/T和11039G/A多态性分布,并用全自动生化测定仪测定总胆固醇、三酰甘油、高密度脂蛋白、低密度脂蛋白、Apo A、Apo B水平。结果 7673C/T在脑梗死组出现突变基因型和等位基因的频率均高于对照组(P<0.05);两组11039G/A基因型频率差异无统计学意义(P>0.05),脑梗死组出现突变A等位基因的频率高于对照组(P<0.05);Logistics回归显示7673C/T CT基因型是脑梗死的危险因素。结论 Apo B基因7673C/T CT基因型是脑梗死的危险因素,而11039G/A多态性与脑梗死无关。
Objective To analyze the relationship between apolipoprotein B gene （7673C/T and l1039G/A） polymorphism and cerebral infarction （CI）. Methods ApoB gene 7673C/T and 11039G/A polymorphisms in 90 CI patients and 82 normal controls were measured by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. TC, TG, HDL, LDL, ApoA and ApoB levels were determined by biochemistry determinator. Results Mutant genotype and allele frequencies of ApoB gene 7673C/T polymorphisms in CI group were significantly higher than those of control group （P 〈 0.05, respectively）, and the frequency of the mutation of A allele in the CI group was also significantly higher （P 〈 0.05）. However, there was no significant difference in the frequency of mutation in ApoB gene 11039G/A between the two groups （P 〉 0.05）. Logistics regression analysis revealed that 7673C/T CT genotype was a risk factor for cerebral infarction. Conclusion The 7673C/T polymorphism may be a risk factor for CI, while 11039G/A polymorphism may not be.
作者
薛慧
陈乃耀
张海霞
赵俊暕
XUE Hui, CHEN Naiyao, ZHANG Haixia, ZHAO Junjian(Department of Hematology, North China University of Science and Technology Affiliated Hospital, Tangshan 063000, Hebei Province, Chin)
出处
《解放军医学院学报》
CAS
2018年第3期248-250,258,共4页
Academic Journal of Chinese PLA Medical School
关键词
载脂蛋白B
基因多态性
聚合酶链反应
脑梗死
apolipoprotein B
gene polymorphism
polymerase chain reaction
cerebral infarction
