摘要
以经破碎处理后的大豆豆瓣为研究对象,经加热软化后通过饱和蒸汽湿热处理,研究该过程对大豆豆瓣中磷脂酶D的影响,以降低浸出毛油中非水化磷脂的含量。大豆豆瓣的软化温度为65℃,软化20 min后,采用响应面法Box-Behnken优化饱和蒸汽处理最佳参数,发现豆瓣破碎为6瓣、饱和蒸汽温度为110℃、通汽时间为2 min时,大豆豆瓣的磷脂酶D活性为0.54 U/mg·min,此时大豆豆瓣中蛋白的氮溶解指数为87.2%,经萃取制得大豆毛油中非水化磷脂含量占总磷脂量2.3%,经水化脱胶油脂精炼率提高了0.36%。
The soybean after crushing treatment was studied. After heating and softening, it was treated by saturated steam and wet heat. The effect of this process on phospholipase D in soybean watercress was studied. The softening temperature of soybean watercress was 65℃, time was 20 min. Response surface methodology （ Box - Behnken） was used to optimize the optimum parameters of saturated steam treatment. It was found that the watercress was broken into 6 petals, the saturated steam temperature was 110 ℃, and time was 2 min. The activity of phospho- lipase D in soybean watercress was 0.54 U/mg - min. The nitrogen soluble index of soybean protein in soybean watercress was 87.2% , the total content of non - hydrated phospholipids in soybean oil was 2.3% , and the rate of oil refining increased by 0.36%.
作者
李婷婷
王旭
刘芳
张鲁
刘天一
于殿宇
Li Tingting, Wang Xu ,Liu Fang, Zhang Lu, Liu Tianyi ,Yu Dianyu(College of Food Science and Technology, Northeast Agricultural University, Haerbin 15003)
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2018年第3期64-69,77,共7页
Journal of the Chinese Cereals and Oils Association
基金
"十三五"国家科技攻关(2016YFD0401402)
国家自然科学基金(31571880)
哈尔滨市科技创新人才(2015RAXXJ008)
关键词
饱和蒸汽
大豆豆瓣
湿热处理
磷脂酶D活性
非水化磷脂
得率
saturated steam, soybean watercress, heat moisture treatment, phospholipase D activity, non -hydrated phospholipids, yield
