摘要
目的 探讨细胞凋亡在反复高 +GZ暴露所致脑损伤中的病理作用以及碱性成纤维细胞生长因子 (b FGF)和丹参对反复高 +GZ暴露致脑损伤的防护作用及其机制。 方法 2 0只 SD大鼠随机分成 5组 ,对照组大鼠 G值为 +1GZ,实验组大鼠在动物离心机上经历了 3次 +14GZ/4 5 s(两次间间隔 30 m in)作用。 b FGF处理组、丹参处理组和生理盐水处理组在 +GZ暴露前后分别给予腹腔注射 b FGF(10 0 μg/kg)、丹参 (15 g/kg)或生理盐水 (2 m l)各一次。于暴露后 6 h处死大鼠取脑 ,用半定量反转录聚合酶链反应 (RT- PCR)方法检测大鼠脑组织中 bcl- 2和 p5 3m RNA表达以及用原位末端脱氧核苷酸转移酶 (Td T)标记法 (TUNEL )检测脑内神经元细胞凋亡情况。 结果 +GZ暴露组可见明显的 bcl- 2、p5 3m RNA表达变化和部分神经细胞凋亡 ,而 b FGF和丹参能阻断 bcl- 2和 p5 3表达变化和抑制神经细胞的凋亡。 结论 +GZ重复暴露可引起大鼠脑神经细胞凋亡及凋亡相关基因 bcl- 2和 p5 3的表达变化 ,细胞凋亡是反复高 +GZ暴露致脑损伤的机制之一 ,而 b FGF和丹参对 +GZ暴露所致的脑损伤具有防护作用。
Objective To investigate the role of apoptosis in mechanisms of brain damage induced by repeated +G Z exposures, and to study the prevention and treatment effect of basis fibroblast growth factor (bFGF) and Radix Salviae Miltiorrhizae (RSM) on the brain injury induced by repeated +G Z exposures. Methods Twenty concious SD rats were randomly divided into five groups. Rats in the control group ( n =4) were exposed to +1G Z and rats in the 4 experimental groups ( n =16) were exposed to +14 G Z for three times, each for 45 seconds with 30 min interval in between. All the +G Z exposured were on an animal centrifuge. bFGF, RSM or NS were injected intraperitoneally into SD rats before and after repeated +G Z exposures. The mRNA expression levels of bcl 2 and p53 in rat brain were measured by semi quantitative reverse transcription polymerase chain reaction (RT PCR), and the apoptotic cell was detected by terminal deoxynuleotidtl transferase mediated dUTP nick end labeling (Tunel) technique. Results The mRNA expression changes of bcl 2 and p53 and apoptotic cells were observed in rat brain taken 6 h after repeated +G Z exposures, but bFGF and RSM could block the expression changes of bcl 2 and p53 and inhibit the cell apoptosis. Conclusion It suggests that apoptosis and expression changes of bcl 2 and p53 in rat brain can be induced by repeated +G Z exposures and the apoptosis is one of the molecular mechanisms of brain damage induced by repeated +G Z exposures. bFGF and RSM were of the prevention and treatment effect on the brain injury induced by repeated +G Z exposures.
出处
《空军总医院学报》
2000年第3期130-133,共4页
Journal of General Hospital of Air Force,PLA
基金
全军医药卫生科研基金!课题 ( 98D0 3 3 )
空军后勤部科研基金!课题 ( KH99190 76)
