摘要
目的:比较正常及炎症来源的牙周膜干细胞(periodontal ligament stem cells,PDLSCs)中线粒体融合蛋白-1(mitofusion 1,Mfn1)的表达差异及其对PDLSCs成骨分化的影响。方法:分离培养PDLSCs,分别为正常来源(HPDLSCs)、牙周炎来源(P-PDLSCs)及应用IL-1β模拟炎症微环境诱导牙周膜干细胞(H-PDLSCs+IL-1β)。RT-PCR方法检测对比H-PDLSCs与P-PDLSCs、H-PDLSCs与H-PDLSCs+IL-1β中Mfn1表达差异;RT-PCR检测成骨基因、茜素红染色及氯化十六烷基吡啶定量,对比P-PDLSCs及si Mfn1下调Mfn1表达后的成骨分化能力。结果:与H-PDSLCs组相比,P-PDSLCs组及H-PDLSCs+IL-1β组中Mfn1表达量显著升高(P<0.05);si Mfn1下调P-PDSLCs中Mfn1表达量后,其成骨分化能力增强(P<0.05)。结论:炎症微环境下,PDLSCs中Mfn1表达量升高,导致成骨分化能力下降。
Objective： To detect the expression of mitofusion-1 （Mfnl） in periodontal ligament stem cells （PDLSCs） isolated from healthy and periodontitis tissue and to study the effect of Mfnl on the osteogenic differentiation of PDLSCs. Methods： PDLSCs were isolated from the healthy and periodontitis human samples （H-PDLSCs and P-PDLSCs）. IL-1β was applied to mimic the inflammation microenvironment（ H-PDLSCs ＋ IL-1 β ）. RT-PCR was used to detect the expression of Mfnl in H- PDLSCs, P-PDLSCs and H-PDLSCs ＋ IL-1β. The expression of Mfnl in P-PDLSCs was down-regulated by siRNA of Mfnl （siMfnl）. The osteogenic differentiation of the cells was examined by RT-PCR, alizarin red staining and cetyl pyridine chlo- ride quantitative analysis. Results： The expression level of Mfnl in P-PDLSCs and H-PDLSCs ＋ IL-1β （5 ptg/ml） groups was higher than that in H-PDLSCs（P 〈0.05）. When the expression of Mfnl in P-PDLSCs was down-regulated by siMfnl the os- teogenic differentiation ability of P-PDSLCs was restored（ P 〈 0.05 ）. Conclusion： Inflammation may promote Mfnl expression in PDESCs and inhibite the osteogenic differentiation of P-PDLSCs.
作者
翟启明
李蓓
刘露
张青
金钫
ZHAI Qiming1 , LIBel2, LIU LuI, ZHANG Qing1, JIN Fang(. 1. 710032 Xi＇an, State Key Laboratory of Military Stomatology ＆ National Clinical Research Center for Oral Diseases ＆ Shaanxi Clinical Research Cen- ter for Oral Diseases, Department of Orthodontic, 2. Department of Histopathology, School of Stomatology, The Fourth Military Medical University, Chin)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2018年第2期172-177,共6页
Journal of Practical Stomatology
基金
国家自然科学基金(编号：81570976)
陕西省青年人才项目(编号：2016JQ8019)
关键词
牙周膜干细胞
炎症微环境
线粒体融合蛋白-1(Mfnl)
成骨分化
Periodontal ligament stem cells （PDLSCs）
Inflammatory microenvironment
Mitofusin-1（Mfnl）
Osteogenic differentiation
