摘要
目的:研究白芍总苷(TGP)对脑缺血再灌注损伤大鼠血液流变学指标的影响,探讨TGP对脑缺血再灌注损伤的保护机制。方法:100只SD大鼠均分假手术组、模型组及TGP低、中、高剂量组,模型组及TGP低、中、高剂量组采用线栓法制备局灶性脑缺血再灌注大鼠模型,假手术组仅做手术、不阻断血流;造模后,TGP低、中、高剂量组分别给予50、100及200 mg/(kg·d)TGP灌胃,假手术组和模型给予等量生理盐水灌胃,1次/d,连续28 d;最后1次灌胃后,各组大鼠进行盲法神经功能评分,测定大鼠脑组织含水量及脑组织梗死体积,HE染色观察大鼠海马CA1区病变,测定大脑组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量,测定大鼠全血黏度(低切、中切、高切)、血浆黏度及血沉,并计算红细胞聚集指数、红细胞变性指数及红细胞刚性指数、红细胞压积。结果:与假手术组大鼠比较,模型组大鼠神经功能评分、脑组织含水量、梗死体积、MDA含量显著升高,全血黏度(低、中、高切)、血浆黏度、红细胞聚集指数、刚性指数、红细胞压积及血沉均显著升高,脑组织SOD、CAT活性显著降低,红细胞变形指数显著降低(P<0.05或P<0.01);与模型组比较,TGP中、高剂量组神经功能评分、脑组织含水量、梗死体积、MDA含量均显著降低(P<0.05或P<0.01),全血黏度(低、中、高切)、血浆黏度、红细胞聚集指数、刚性指数、红细胞压积及血沉均显著降低,脑组织SOD、CAT活性显著升高,红细胞变形指数显著升高(P<0.05或P<0.01);假手术组大鼠海马CA1区神经元未见异常,模型组大鼠海马CA1区神经元形态结构呈现明显异常,TGP各剂量组大鼠海马CA1区神经元病变较模型组有不同程度的减轻,并呈现一定的剂量依赖性。结论:TGP可能通过改善血液流变学而对脑缺血再灌注损伤起到一定的保护作用。
Objective： To study the effect of total glucosides of paeony（ TGP） on hemorheology index in rats with cerebral ischemia-reperfusion and explore the protective mechanism of total glucosides of paeony on cerebral ischemia-reperfusion injury. Methods： The models of focal cerebral ischemic reperfusion were established with suture method and were randomly divided into model group,TGP low,medium,high-dose（ 50,100,200 mg/kg） groups; and set sham operation group（ n = 20）.The drugs were administered by gavage for 28 days,once a day,rats in model group and sham operation group were given normal saline instead. After 28 d,the neurological function were evaluated by blind method,infarct volume and water content of the brain tissue were detected,brain tissue lesions of CA1 area in the hippocampus was observed through HE staining. Superoxide dismutase（ SOD）,catalase（ CAT） activity and the content of malondialdehyde（ MDA） in brain tissue were determined.Whole blood viscosity,plasma viscosity and erythrocyte sedimentation rate were detected,and the erythrocyte aggregation index,erythrocyte degeneration index,erythrocyte rigidity index,hematocrit were calculated. Results： Compared with sham operation group,neurological function scores,the water content,the infarct volume and the content of MDA,whole blood viscosity,plasma viscosity,erythrocyte aggregation index,rigidity index,hematocrit and erythrocyte sedimentation rate of rats in model group increased significantly,erythrocyte deformation index,SOD and CAT activity decreased significantly（ P 0. 05 or P 0. 01）; Compared with model group,neurological function scores,the water content,the infarct volume and the content of MDA,whole blood viscosity,plasma viscosity,erythrocyte aggregation index,rigidity index,hematocrit and erythrocyte sedimentation rate of rats in TGP medium,high-dose（ 50,100,200 mg/kg） groups decreased significantly,erythrocyte deformation index,SOD and CAT activity increased significantly（ P 0. 05 or P 0. 01）. The neurons in hippocampal CA1 area of the sham operation group were not abnormal. The morphology and structure of neurons in the hippocampal CA1 area of the model group showed significant abnormality. The neuron charges in the hippocampal CA1 area of TGP groups were reduced to different degrees compared with the model group,showing a dose-dependent manner. Conclusion TGP may play a protective role on cerebral ischemia-reperfusion injury by improving hemorheology.
作者
张玥
朱晓冉
ZHANG Yue, ZHU Xiaoran(Department of Pharmacy, People＇s Hospital of Hebei Province, Shijiazhuang 050051, Hebei, Chin)
出处
《贵州医科大学学报》
CAS
2018年第3期305-309,共5页
Journal of Guizhou Medical University
基金
廊坊市科技支撑计划项目(2016013151)
关键词
白芍总苷
脑缺血再灌注
血液流变学
神经功能
海马CA1区
total glucosides of paeony
cerebral ischemia-reperfusion
hemorheology
nerve function
hippocampal CA1 area
