摘要
目的研究沙利度胺联合顺铂对人宫颈癌Hela细胞增殖及迁移能力的影响。方法培养人宫颈癌Hela细胞,分为顺铂组(A组给予1 mg/L的顺铂进行干预)和不同浓度沙利度胺联合顺铂组(B、C、D组),分别进行100 mg/L、200 mg/L、400 mg/L的沙利度胺和1 mg/L的顺铂临床干预,测定细胞增殖、细胞迁移、侵袭恶性生物学行为情况。结果D组药物作用12 h、24 h、36 h、48 h时,细胞的抑制率明显高于A、B、C组,B、C、D组Hela细胞抑制率依次升高。D组药物作用12 h、24 h、36 h、48 h时,细胞的迁移率、细胞的穿膜数均明显低于A、B、C组,B、C、D组Hela细胞迁移率及细胞穿膜数依次降低,且组间差异均有统计学意义(P<0.05)。结论沙利度胺联合顺铂有助于抑制Hela细胞增殖和迁移。
Objective To study the effect of thalidomide combined with cisplatin on human cervical cancer Hela cell proliferation and migration. Methods Human cervical cancer Hela cells were cultured and divided into cisplatin group（ group A treated with 1 mg/L cisplatin intervention） and different concentrations of thalidomide combined with cisplatin（ B,C,D group）,respectively,and clinical intervention was performed with 100 mg/L,200 mg/L,400 mg/L of thalidomide and 1 mg/L of cisplatin. Cell proliferation,cell migration,invasion and malignant biological behavior were determined. Results The inhibitory rates of cells in group D were significantly higher than those in groups A,B and C at 12 h,24 h,36 h and 48 h after treatment.The inhibitory rates of Hela cells in groups B,C and D increased in turn. In group D,the cell migration rate and cell penetrating number at 12 h,24 h,36 h,48 h after treatment were significantly lower than those in groups A,B,C,and that and cell penetrating number in B,C and D decreased in turn,and the differences between groups were statistically significant（ P〈0. 05）. Conclusion Thalidomide combined with cisplatin is helpful to inhibit the proliferation and migration of Hela cells.
作者
申自玮
SHEN Zi - wei(Department of Gynecology and Obstetrics, Handan Central Hospital, Handan, Hebei 056008, Chin)
出处
《中国卫生检验杂志》
CAS
2018年第4期453-455,共3页
Chinese Journal of Health Laboratory Technology
