摘要
目的:探讨脂多糖(lipopolysaccharide,LPS)对多发性骨髓瘤细胞增殖的影响及其作用机制。方法:采用LPS作用于多发性骨髓瘤细胞RPMI 8226后,通过MTT法检测细胞的增殖情况,流式细胞术检测细胞周期的变化,Western blot检测NF-κB信号通路中相关蛋白p65和IκB-α的表达情况。结果:随着LPS作用浓度(0-8μg/mL)的增加,RPMI 8226细胞增殖活力呈先上升后下降的趋势,在2μg/m L LPS作用下,RPMI8226细胞的增殖活力最显著(P<0.05)。相同浓度的LPS诱导RPMI8226细胞不同时间,其细胞增殖呈时间依赖性。流式细胞术检测结果显示:不同浓度LPS(0.5、1、2μg/mL)作用RPMI 8226细胞后,G0/G1期细胞的比例明显低于对照组(P<0.05),S期和G2/M期细胞比例明显增加(P<0.05);Western blot检测结果显示:2μg/mL的LPS处理的RPMI8226细胞p65和IΚB-α蛋白的表达明显高于对照(P<0.05)。结论:低浓度LPS能够诱导多发性骨髓瘤细胞RPMI 8226的增殖,其作用机制可能与上调信号通路NF-κB中相关蛋白p65和IκB-α有关。
Objective： To investigate the effect of lipopolysaccharide（LPS） on the proliferation of multiple myeloma cells and its mechanism. Methods： RPMI 8226 cells were treated by LPS, the proliferation of cells was determined by MTT method, the changes of cell cycle were measured by flow cytometry, and the expressions of protein p65 and IκB-alpha were determined by Western blot. Results：With the increase of LPS concentration（0 to 8 μg/mL）, the proliferation activity of RPMI 8226 cells treated by different concentrations of LPS was firstly increased and then decreased, and the proliferation activity of RPMI8226 cells was the highest at 2 μg/mL LPS treatment（P〈0.05）. The proliferation of RPMI8226 cells was in a time dependent manner. Flow cytometry showed that, the proportion of G0/G1 cells were significantly lower and the proportion of cells in S phase and G2/M were significantly increased in different concentrations of LPS（1 μg/mL, 0.5 μg/mL and 2 μg/mL）-treated RPMI 8226 cells than that of the control group（P〈0.05）. The result of Western blot showed that the protein expressions of p65 and the IκB-alpha in 2 μg/mL LPS-treated RPMI 8226 cells were significantly higher than those of the control group（P〈0.05）. Conclusion： Low concentration of LPS can induce the proliferation of multiple myeloma cell RPMI8226, whih may be related to the upregulation of p65 and I kappa B-alpha protein.
出处
《现代生物医学进展》
CAS
2017年第36期7017-7021,共5页
Progress in Modern Biomedicine
基金
陕西省科学技术研究发展计划项目(2014K11-02-03-03)
