摘要
目的 :检测基质金属蛋白酶 3(MMP- 3)在大鼠正常动脉及腹动脉瘤模型组织中的表达 ,以探讨其在腹主动脉瘤发病机制中的作用。方法 :建立大鼠腹主动脉瘤灌注模型 ,应用免疫组织化学和分子原位杂交技术检测 MMP- 3的表达。结果 :正常动脉壁组织 MMP- 3蛋白表达为弱阳性或阴性 ,而动脉瘤组织中 MMP- 3蛋白表达显著增高 ,阳性率为 8/ 8;MMP- 3阳性染色主要位于中膜的平滑肌细胞和外膜的炎症细胞 ,其中炎症细胞浸润的区域染色明显增强。 MMP- 3m RNA在动脉瘤组织中呈阳性表达 ,在动脉外膜炎症细胞浸润的区域和小血管聚集区呈强阳性 ;对照组动脉组织未见 MMP- 3m RNA阳性表达。 结论 :腹主动脉瘤组织中 MMP- 3蛋白及 m RNA较正常动脉组织表达显著增高 ,尤其在炎症细胞浸润的区域表达明显增强 ;提示 MMP-
Objective:To investigate matrix metalloproteinase 3 (MMP 3) expression in experimental abdominal aortic aneurysms (AAA) rat model. Methods:The expression of MMP 3 were observed in 8 rat AAA models and 8 normal control aortic tissues using immunohistochemistry and molecular in situ hybridization technique. Results:The positive rate of MMP 3 protein expression was 8/8 in AAA and it was markedly increased compared with that of control samples( P <0.01). The positive expression of MMP 3 protein, mostly in adventitia inflammatory infiltration areas and media vascular smooth muscle cells in AAA model tissues, was much stronger than that of control aortic tissues. The expression of MMP 3 mRNA in AAA group was much stronger than that in control group. The expression pattern of MMP 3 mRNA in AAA was consistent with expression of MMP 3 protein. Conclusion:The protein and mRNA expression of MMP 3 is markedly increased in experimental AAA than in control abdominal aortic tissues. MMP 3 may be a key initial factor in experimental AAA formation and progression.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2002年第8期877-879,共3页
Academic Journal of Second Military Medical University
基金
军队杰出人才基金资助项目 (98J0 0 5 )
上海市卫生系统百名跨世纪优秀学科带头人培养计划基金(97BR0 4 7)
上海市科技发展基金攻关计划项目(0 0 4 4 190 2 9)
长海医院学科攀登计划基金资助课题
关键词
基质金属蛋白酶3
大鼠
腹主动脉瘤
免疫组织化学
原位杂交
aortic aneurysm,abdominal
metalloproteinases
immunohistochemistry
in situ hybridization
model,cardiovascular
