摘要
在室温条件下利用反相微乳液法一步制备智能凝胶生物酶反应器。研究发现,该方法制备的智能凝胶生物酶反应器具有多重响应性能;偏光显微镜观察发现,合成的微凝胶粒子共价固定漆酶将影响微凝胶粒子的粒径,其体积相转变温度将向高温方向移动,而pH值响应性减弱。凝胶粒子共价包埋固定漆酶有效地抑制了酶蛋白的逸出,逸出量降低近20%。反相微乳液一步法凝胶粒子共价包埋固定漆酶对染液的脱色率可达60%,并且在多次循环使用后依然具有良好的脱色效果。
The inverse emulsion polymerization is applied to one-step synthesis of smart microgel en- zyme reactor under room temperature. The result shows that the smart microgel enzyme reactor has multiple response capabilities. Polarizing microscope shows that the diameter of the immobilization of laccase within microgel by covalent bond is decreased compared with microgel. Meanwhile, Tvpt （vol- ume phase transition temperature） of microgel shifts to higher temperature after immobilized laccase and pH value response performance becomes weaker. Leaching rate of laccase coupling to microgel is reduced by nearly 20% compared to laccase encapsulating in microgel. In addition, microgel immobilized taccase shows 60% decolorization rate and it can remain high decolrization rate after recyling.
出处
《印染》
北大核心
2018年第4期15-19,共5页
China Dyeing and Finishing
基金
国家级大学生创新训练项目资助项目(201710354007)
关键词
印染废水
脱色
反相微乳液法
智能凝胶酶反应器
一步法
合成
dyeing wastewater, decolorization
inverse emulsion polymerization
smart microgel enzymereactor
one-step
synthesis
