摘要
目的探讨CD82在口腔鳞癌OSCC-25细胞的表达情况及过表达CD82基因对口腔鳞癌细胞增殖和侵袭、细胞凋亡的影响。方法将真核表达载体p IRES2-EGFP-CD82转染至口腔鳞癌OSCC-25细胞中,分别培养0,12,24,48,72 h,并在各个时点采用Western blot观察CD82蛋白表达情况并确定表达效率最高时间。同时以未转染的OSCC-25细胞为空白对照组,OSCC-25细胞加入空白质粒为阴性对照组,实验分别通过MTT,Transwell小室及AnnexinⅤ-FITC/PI双染细胞凋亡检测方法检测其对OSCC-25细胞增殖、侵袭及细胞凋亡的影响。结果转染p IRES2-EGFP-CD82真核表达质粒载体72 h,Western blot结果显示CD82蛋白水平明显增高(P<0.05)。与空白及阴性组相比较,实验组OSCC-25细胞的增殖、侵袭能力均受抑制,细胞凋亡水平上升,且差异具有统计学意义(P<0.05)。结论在体外活细胞实验中,过表达CD82基因可有效抑制口腔鳞癌OSCC-25细胞的增殖和侵袭能力,并促进鳞癌细胞的凋亡。
Objective To investigate the protein levels of CD82 and explore the effects of its overexpression on OSCC-25 cell proliferation,invasion and apoptosis. Methods The eukaryotic expression vector p IRES2-EGFP-CD82 was transfected into OSCC-25 cells.The protein levels of CD82 were examined using Western blot at 0 h,24 h,48 h and 72 h after transfection. The non-transfected cells were chosen as blank group and the cells transfected with the NC plasmid group were chosen as negative control group. Cell proliferation,invasion and apoptosis were assessed by MTT,Transwell and Annexin Ⅴ-FITC/PI staining,respectively. Results When the eukaryotic plasmid p IRES2-EGFP-CD82 was transfected at 72 h,the expression of CD82 protein was highly increased using Western blot analysis（ P 〈0. 05）. Compared to blank group and negative group,the proliferation and invasion of OSCC-25 cells were significantly inhibited in CD82 transfection group,and the apoptotic rate of OSCC-25 cells was increased（ all P 〈0. 05）. Conclusion The overexpression of CD82 may inhibit the proliferation and invasion of OSCC-25 cells,but enhance the apoptotic of OSCC-25 cells in vitro.
出处
《山西医科大学学报》
CAS
2018年第2期123-127,共5页
Journal of Shanxi Medical University
基金
国家自然科学基金资助项目(81072230)
西安医学院博士启动基金资助项目(2016D0C20)
