摘要
目的研究虎杖苷对卵巢摘除骨质疏松大鼠β-Catenin及GSK-3β蛋白表达的影响,探索其对卵巢摘除骨质疏松大鼠的保护作用及可能的作用机制。方法 SPF级♀大鼠60只,随机分为假手术组、模型组、虎杖苷低(5 mg·kg^(-1)·d^(-1))、中(15 mg·kg^(-1)·d^(-1))、高剂量组(45 mg·kg^(-1)·d^(-1)),每组12只,灌胃给药。除假手术组外,其余各组采用摘除大鼠双侧卵巢的方法模拟骨质疏松模型。12周后,用双能X线吸收法测量骨密度(bone mineral density,BMD),ELISA法检测E2、ALP、TRAP等生化指标的水平;Western blotting法检测β-Catenin和GSK-3β蛋白的表达,q RTPCR法检测β-Catenin和GSK-3βm RNA的表达。结果与模型组比较,虎杖苷各组BMD,E2显著升高(P<0.01)、血清ALP、TRAP显著降低(P<0.01);β-Catenin蛋白的表达升高(P<0.01),GSK-3β表达降低(P<0.01);β-Catenin m RNA表达显著升高(P<0.01),GSK-3βm RNA表达显著降低(P<0.01)。结论虎杖苷对去卵巢骨质疏松有一定的治疗作用,其机制可能与增加β-Catenin表达,减少GSK-3b表达有关。
OBJECTIVE To study the effect of polydatin（PD） on the expression of β-Catenin and GSK-3β protein in ovariectomy osteoporosis rats. METHODS The 60 female SPF rats were randomly divided into sham operation group, model group, PD low（5 mg·kg^（-1）·d^（-1））, middle（15 mg·kg^（-1）·d^（-1）） and high dose（45 mg·kg^（-1）·d^（-1）） groups（12 rats in each group, ig）, every group were established by ovariectomy except sham group. After 12 weeks, the bone mineral density（BMD） of these rats was detected by dual energy X-ray absorptionmetry, ELISA method was used to detect serum estradiol（E2）, bone alkaline phosphatase（ALP）, tartrate resistant acid phosphatase（TRAP） and other biochemical indicators of the level; Western blotting was used to detect the expression of β-Catenin and GSK-3β protein; q RTPCR was used to detect the expression of β-Catenin and GSK-3β m RNA. RESULTS Compared with model group, PD groups increased BMD, E2 significantly（P〈0.01）, decreased ALP, TRAP level（P〈0.01） and increased the expression of β-Catenin protein and m RNA（P〈0.01）; decreased the expression of GSK-3β protein and m RNA（P〈0.01）. CONCLUSION PD has therapy effects in ovariectomy osteoporosis rats. Its mechanism could be relative with increasing the expression of β-Catenin protein and decreasing expression of GSK-3β.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2018年第1期103-106,共4页
Chinese Journal of Modern Applied Pharmacy
基金
重庆市科委基础与前沿一般项目(CSTC2016jcyj A0373
CSTC2016jcyj A0268)
