摘要
目的 建立辣木叶通便复方中异槲皮苷、紫云英苷的含量测定方法。方法 采用HPLC法，Cosmosil-C18柱（4.6 mm×250 mm，5 μm），柱温为40 ℃，流动相为B乙腈-A 0.1%磷酸水，检测波长为350 nm，流速为1.3 ml/min。结果 辣木叶通便复方中异槲皮苷和紫云英苷在30 min中分离效果较好，异槲皮苷在0.24～4.73 μg，紫云英苷在0.11～2.19 μg范围内线性关系良好（r＞0.999 0，n＝8）且异槲皮苷和紫云英苷的平均回收率分别为99.09%（RSD为0.60%）和99.08%（RSD为1.37%）。结论 所建立的辣木叶通便复方高效液相色谱法分析方法，可靠、快捷、准确，具有较好的可重复性和稳定性，可为辣木叶通便复方的含量测定提供参考。
Objective To establish an HPLC method for simultaneous determination of Isoquercitrin and astragalin in Moringa oleifera leaves prescription. Methods By using HPLC method with Cosmosil-C18 column （4.6 mm × 250 mm, 5 μm） and the column temperature was 40 degrees Celsius, the mobile phase contains a 0.1% phosphoric acid in water（A） and acetonitrile（B）, the detection wavelength was set at 350nm by UV detector and the flow rate was 1.3 ml/min. Results The Isoquercitrin and astragalin was better separated in 30 minutes, and Isoquercitrin had a good linear correlation at the range of 0.24-4.73 μg, while astragalin was 0.11-2.19 μg, the average spiked recoveries of isoquercetin and astragalin were 99.09% （RSD=0.60%） and 99.08% （RSD=1.37%）, respectively. Conclusions The proposed method is simple,accurate,and repeatable and can be used for quality control as well as a powerful tool to evaluate the processing of Moringa oleifera leaves. It can provide the reference basis and data support for the determination of content of Moringa oleifera health products.
出处
《国际中医中药杂志》
2018年第2期153-156,共4页
International Journal of Traditional Chinese Medicine
关键词
色谱法
高压液相
化学定量分析
辣木叶
通便复方
Chromatography
high pressure liquid
Chemical quantitative analysis
Moringa oleifera
Relaxing bowels compounds
